厄贝沙坦对实验性糖尿病大鼠肾脏保护作用及对ICAM-1与VCAM-1表达的影响

目的:从影响细胞间粘附分子-1(ICAM-1)和血管细胞粘附分子-1(VCAM-1)表达的角度,探讨厄贝沙坦对实验性糖尿病大鼠肾脏的保护作用及其可能机制。方法:Wistar大鼠24只,随机分为对照组、模型组、药物组,每组8只。链脲佐菌素(STZ)诱导糖尿病大鼠模型,药物组大鼠每日给予厄贝沙坦150mg/kg灌胃,持续8周。8周后抽血测空腹血糖(FBG)、糖化血红蛋白(HbA1c)、尿素氮(BUN)、肌酐(Scr);测定24h尿微量白蛋白(mAlb),计算尿白蛋白排泄率(UAER)。处死Wistar大鼠,取其肾脏,计算肾脏肥大指数,用免疫组化方法检测ICAM-1,蛋白质印迹法(Westernblot)检测VCAM-1蛋白表达水平。结果:模型组和药物组的FBG、HbA1c、BUN、Scr均高于对照组,上述指标在模型组与药物组之间无统计学差异。模型组UAER高于对照组,而药物组则低于模型组。药物组ICAM-1与VCAM-1蛋白表达量低于模型组。结论:厄贝沙坦对实验性糖尿病大鼠的肾脏有保护作用,其机制可能是通过抑制ICAM-1与VCAM-1在肾脏的表达来实现的。

Protective Effects of Irbesartan on the Kidneys of Diabetic Rats and Expressions of ICAM-1 with VCAM-1

Objective:To investigate the protective effects of Irbesartan on the kidneys of diabetic rats,and study the possible mechanisms related to the expressions of intercelluar adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule-1(VCAM-1).Method:Twenty-four male Wistar rats were randomly and equally divided into control group,model group and treatment group.There were eight rats in every group.The rat model of DN was induced by injection of streptozocin(STZ).The rats of treatment group were fed with irbesartan.Eight weeks later,BG,HbA1c,BUN,Scr,mAlb,UAER were measured.The expression of ICAM-1 was detected by immunohistochemistry.The expression of VCAM-1 was detected by western blot.Results:The levels of BG,HbA1c,BUN and Scr in model group were higher than those in control group.Compared with model group,the levels of them did not decrease significantly in treatment groups.UAER in model group was hingher than that in treatment group.The expression of ICAM-1 and VCAM-1 in treatment group were decreased significantly as compared with those of model group.Conclusion:Irbesartan seems to have renal protective effects on kidney of diabetic rats.This effect may be achieved by decreasing the expressions of ICAM-1 and VCAM-1.