Increased muscarinic cholinergic receptor density on CD4 lymphocytes in progressive multiple sclerosis |
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Authors: | Banu Anlar Joseph W. Karaszewski Anthony T. Reder Barry G.W. Arnason |
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Affiliation: | Department of Neurology, University of Chicago, IL 60637. |
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Abstract: | We measured the density and affinity of muscarinic cholinergic receptors (MR) in 29 chronic progressive and ten stable multiple sclerosis (MS) patients and 27 control subjects using [3H]N-methyl-scopolamine. The density of MR on CD4+ lymphocytes was significantly higher in chronic progressive MS (CPMS) than in controls (7.9 +/- 0.7 vs. 4.5 +/- 0.4 fmol/10(6) cells, p less than 0.001). Stable patients did not differ significantly from control subjects. Receptors of the M1 subtype were measured on CD4+ lymphocytes of nine patients and seven controls with the selective antagonist [3H]methylpirenzepine: M1/total receptor ratio was 64.1% in CPMS and 81.2% in controls, suggesting a selective increase of M2-type MR in CPMS. The findings may relate to parasympathetic denervation hypersensitivity of lymphocytes or to lymphocyte activation which is known to be associated with increased MR number. |
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Keywords: | Multiple sclerosis Muscarinic receptor Lymphocyte [3H]N-Methylscopolamine [3H]Pirenzepine |
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