蟾皮中蟾毒配基类成分的分离与鉴定

目的研究中华大蟾蜍(Bufo bufo gargarizansCantor)皮中的蟾毒配基类成分。方法利用反复硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备高效液相色谱、重结晶等手段分离纯化蟾皮中的蟾毒配基类成分,根据化合物的理化性质和各种波谱学数据鉴定其化学结构。结果分离得到11个蟾毒配基类化合物,分别鉴定为脂蟾毒配基(resibufogenin,1)、华蟾毒精(cinobufagin,2)、蟾毒灵(bufalin,3)、远华蟾毒精(telocinobufagin,4)、蟾毒它灵(bufotalin,5)、去乙酰华蟾毒它灵(desace-tylcinobufotalin,6)、嚏根草苷元(hellebrigenin,7)、沙蟾毒精(arenobufagin,8)、日蟾毒它灵(gam-abufotalin,9)、11β-羟基脂蟾毒配基(11β-hydroxylresibufogenin,10)、华蟾毒它灵(cinobufotalin,11)。结论化合物10和11为首次从中华大蟾蜍皮中分离得到。

Isolation and identification of bufogenin constituents from the skin of Bufo bufo gargarizans Cantor

Objective To study the bufogenin constituents from the skin of Bufo bufo gargarizans Cantor. Methods Repeated silica gel column, ODS, HPLC, Sephadex LH-20, and recrystallization were used to isolate and purify the bufogenins constituents. Their physico-chemical properties and the spectral analysis were used to identify the structures. Results Eleven bufogenin compounds were isolated and identified from the skin of Bufo bufo gargarizans Cantor and identified as resibufogenin(1), cinobufagin(2), bufalin(3), telocinobufagin(4), bufotalin(5), desacetylcinobufotalin(6), hellebrigenin(7), arenobufagin(8), gamabufotalin(9), 11β-hydroxylresibufogenin(10), cinobufotalin(11). Conclusion Compounds 10 and 11 are obtained from the skin of Bufo bufo gargarizans Cantor for the first time.