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| 人肺癌相关单克隆抗体的制备及其抗原的纯化 | |
| 引用本文： | 姚晓玲,柳晓燕,吴强,徐振山,宋礼华. 人肺癌相关单克隆抗体的制备及其抗原的纯化[J]. 中国免疫学杂志, 2006, 22(12): 1140-1142,1145 |
| 作者姓名： | 姚晓玲 柳晓燕 吴强 徐振山 宋礼华 |
| 作者单位： | 1. 安徽医科大学生物化学与分子生物学教研室,合肥,230032 2. 安徽大学生命科学院,合肥,230039 3. 安徽医科大学病理学教研室,合肥,230032 4. 安徽安科生物工程(集团)股份有限公司,合肥,230088 |
| 摘 要： | 目的：研制抗人肺癌细胞（A549）单克隆抗体，并对其所识别的抗原进行免疫亲和层析纯化。方法：以人肺癌细胞系A549免疫BALB／c小鼠，常规融合，以间接ELISA筛选，免疫组化研究单克隆抗体的特性，通过免疫亲和层析纯化所识别相关的抗原。结果：成功获得了一株能够稳定分泌抗人肺癌相关抗原单克隆抗体的杂交瘤细胞株289，并提取了其识别的相关抗原。结论：2139单克隆抗体及其所识别抗原有可能进一步用于肺癌的实验室诊断。 |
| 关 键 词： | 单克隆抗体 肺癌 免疫亲和层析 |
| 文章编号： | 1000-484X（2006）12-1140-04 |
| 收稿时间： | 2006-06-14 |
| 修稿时间： | 2006-06-142006-08-17 |
Preparation of monoclonal antibody against human lung cancer and purification of its antigen by the immunoaffinity chromatography |
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| YAO Xiao-Ling,LIU Xiao-Yan,WU Qiang,XU Zhen-Shan,SONG Li-Hua. Preparation of monoclonal antibody against human lung cancer and purification of its antigen by the immunoaffinity chromatography[J]. Chinese Journal of Immunology, 2006, 22(12): 1140-1142,1145 | |
| Authors: | YAO Xiao-Ling LIU Xiao-Yan WU Qiang XU Zhen-Shan SONG Li-Hua |
| Affiliation: | Department of Biochemistry and Molecule Biology, Anhui Medical University, Hefei 230032, China |
| Abstract: | Objective:To produce the mcAb specifically reacting with lung cancer and to purificate its antigen.Methods:The mice were immunized with A549, the mcAb 2B9 was screened by indirect cell ELISA and immunohistochemistry, and its antigen was purificated by immunoaffinity chromatography.Results:A mcAb was obtained, which could react to lung cancer but very little or not to normal lung tissue and other caner tissues, and the antigen of the mcAb was purificated from the cell lysate.Conclusion:A mcAb which can react to lung cancer have been obtained and its antigen was purificated, they may be useful on clinic for diagnosis and prognosis of lung cancer. |
| Keywords: | Monoclonal antibody Lung cancer Immunoatilnity chromatography |
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