| Construction of Antisense MT1-MMP Vector and Its Inhibitory Effects on Invasion of Human Ovarian Cancer Cells |
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| 作者姓名： | 吴明富 史艳燕 奚玲 李琼 廖国宁 韩志强 卢运萍 马丁 |
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| 作者单位： | Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China，Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China，Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China，Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China，Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China，Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China，Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China，Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China |
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| 基金项目： | This study was sapported by grants from National Basic Research program ＂973＂ （No. 2002CB513107）. |
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| 摘 要： | Themetastaticspreadofcancercellsfromaprimarytumortodistantsitesinthebodyisre sponsibleformostcancerpatientmorbidityandmortality.Duringtumormetastasis,thedegrada tionofexteacellularmatrix(ECM)isakeystep.Membrane type1matrixmetalloproteinase(MT1MMP/MMP14)isanewlydiscoveredenzyme,whichplaysacrucialroleintumormetastasisbydirectlybreakingthematrixbyproteolysisinthepericellularspaceandindirectlyactivatingpro MMP21].Atpresent,itiselusivewhetherendog enousexpressionofMT1MMPinhumanovariancance…
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| 关 键 词： | MT1-MMP 卵巢癌 肿瘤细胞 肿瘤入侵 |
| 收稿时间： | 2005-10-26 |
Construction of antisense MT1-MMP vector and its inhibitory effects on invasion of human ovarian cancer cells |
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| Wu Mingfu,Shi Yanyan,Xi Lin,Li Qiong,Liao Guo-Nin,Han Zhi-Qiang,Lu Yuh-Ping,Ma Ding.Construction of Antisense MT1-MMP Vector and Its Inhibitory Effects on Invasion of Human Ovarian Cancer Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(6):715-717. |
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| Authors: | Wu Mingfu Shi Yanyan Xi Lin Li Qiong Liao Guo-Nin Han Zhi-Qiang Lu Yuh-Ping Ma Ding |
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| Institution: | Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan , 430030 , China |
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| Abstract: | Summary Membrane-type 1 matrix metalloproteinase (MT1-MMP/MMP-14) plays crucial roles in tumor cell growth, invasion, and angiogenesis.
To clarify whether the endogenously expressed MT1-MMP in metastatic human ovarian carcinoma cell lines SKOV3 plays a critical
role in tumor cell invasiveness, antisense MT1-MMP cloned in cukaryotic expression vector pMMP14as was transferred into SKOV3
cells. 48h after transfection, decreased expression of endogenous MT1-MMP protein was detected in pMMP14as-transfected SKOV3
cells and the activation of pro-MMP2 was inhibited markedly. The mean percentage of invasive cells was (62.50±5.30)% in pMMP14as-transfected
cells, which was obviously less than that (97.20±6.90) % in the control. Thus, antisense MT1-MMP effectively inhibited the
endogenous MT1-MMP expression and the invasiveness in SKOV3 cells, suggesting that MT1-MMP may be a therapeutic target molecule
for human invasive ovarian cancers.
WU Mingfu, male, born in 1972, M.D., Ph.D.
This study was sapported by grants from National Basic Research program “973” (No.2002CB513107). |
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| Keywords: | membrane-type 1 matrix metalloproteinase (MT1-MMP) ovarian tumor invasiveness |
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