淡竹叶总黄酮对拘束负荷所致小鼠肝损伤的保护作用

目的:通过小鼠拘束应激模型,研究淡竹叶总黄酮对拘束应激负荷小鼠肝损伤的保护作用。方法:利用Hp20大孔吸附树脂对淡竹叶提取物经不同浓度乙醇洗脱后得到淡竹叶总黄酮。观测淡竹叶总黄酮125,250,500mg·kg-1连续ig5d,对拘束应激18h后致急性肝损伤模型小鼠血浆中丙氨酸氨基转移酶(ALT)活性、抗氧化能力指数(ORAC)、肝组织中ORAC、丙二醛(MDA)和一氧化氮(NO)含量的影响。结果:与模型组相比,淡竹叶总黄酮可以明显降低小鼠血浆ALT活性、肝组织的MDA含量和NO含量,显著提高血浆和肝组织的抗氧化能力指数。结论:淡竹叶总黄酮对拘束负荷引起小鼠急性肝损伤有一定的保护作用。

Protective Effects of Herbal Lophatheri Flavonoids on Restraint Stress-induced Liver Damage in Mice

Objective: To investigate the protective effect and the mechanism of Herbal Lophatheri flavonoids (HLF) on mice loaded with restraint stress. Method: The filtrate of extraction of Herbal Lophatheri was adsorbed by Hp20 macroporous exchange resin, eluted by ethanol. After oral administration of HLF 125, 250 and 500 mg·kg^-1 for five days. The mice were restrained for 18 h after 30 min of the last intragastric administration to induce liver injury. The protective effects were evaluated by assessing alanine aminotransferase (ALT) levels in plasma. The contents of NO and malondialdehyde (MDA) in liver were performed respectively. Oxygen radical absorbance capacity (ORAC) assay was used to measure the antioxidant capacity. Results : Compared with the stress model, HLF could decrease activities of ALT in the plasma and the levels of MDA and the contents of NO in liver. And it could significantly increase ORAC activity in the plasma and liver. Conclusion : Herbal Lophatheri flavonoids exert protective effect on liver injury in mice loaded with restraint stress.