Postembedding immunocytochemical GABA labeling in rat neocortex cultures: applicability in quantitative studies |
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| Authors: | B M de Jong H J Romijn R M Buijs |
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| Affiliation: | 1. Program in Neuroscience, Christopher Newport University, Newport News, VA, USA;2. Department of Molecular Biology & Chemistry, Christopher Newport University, Newport News, VA, USA;1. Department of Spine Surgery, Zhongda Hospital, Southeast University, Nanjing 210009, China;2. Department of Orthopaedics and Traumatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, China;3. Department of Spine Surgery, Inner Mongolia People’s Hospital, Hohhot 010017, China;4. Department of Spine Surgery, The Second Affiliated Hospital of Hainan Medical University, Haikou 570216, China;5. Department of Orthopedics, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch, Southeast University, Nanjing 211200, China;1. Institute of Zoology, Rheinische Friedrich-Wilhelms-Universität Bonn, Poppelsdorfer Schloss, Meckenheimer Allee 169, 53115 Bonn, Germany;2. Department of Integrative Biology, Institute for Neuroscience, University of Texas at Austin, 2415 Speedway, Austin, TX 78712, USA |
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| Abstract: | The value of gamma-aminobutyric acid (GABA) immunogold labeling in the quantification of nerve endings was studied in dissociated rat neocortex cultures. Adjacent sections were processed in 3 series according to the same staining protocol. Of all nerve ending profiles examined, 20% appeared to be GABA-positive, while 7% could not be classified. In cultures pretreated with gabaculine, GABA-positive endings were labeled more heavily. Cell bodies could always be discriminated as GABA-positive or GABA-negative. Of all neuronal cell profiles, 14% appeared to be GABA-positive. These neurons could also be identified by light microscopy using the peroxidase-anti-peroxidase method. |
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