不同氧疗技术对急性氮气窒息大鼠治疗效果的评价

目的 评价不同氧疗技术对急性氮气窒息大鼠的治疗效果,以探讨更加适合急性氮气窒息患者的现场氧疗技术.方法 健康成年雄性Wistar大鼠60只,随机分成对照组、单纯染毒组、33%浓度氧吸氧组、50%浓度氧吸氧组和高压氧舱吸氧组,每组12只.将氮气与空气混合成为96%氮气浓度的气体充入染毒罐,染毒组大鼠在染毒罐中静式吸入此混合气体1 h.单纯染毒组大鼠于染毒后吸入常压空气1 h;高压氧治疗组将大鼠置于高压氧舱中,纯氧洗舱并进行高压氧(压力为0.2 MPa,氧浓度>90%)吸氧治疗1 h;其他2个染毒组大鼠分别置于33%氧浓度和50%氧浓度的氧疗罐中,吸氧1 h.比较对照组、单纯染毒组和各吸氧治疗组大鼠的行为学表现、动脉血氧分压(PO2)、二氧化碳分压(PCO2)和血氧饱和度(SPO2)、肝肾功能指标和心肌酶的变化情况.结果 各组大鼠在染毒后出现先兴奋后抑制的表现,氧疗后意识得到恢复.单纯染毒组大鼠PO2(79.67±9.12)mm Hg]和SPO2(94.92±2.78)mm Hg]明显低于对照组,差异有统计学意义(P<0.01);33%氧浓度组、50%氧浓度组、高压氧疗组大鼠PO2分别为(94.75±7.24)、(94.92±8.98)、(104.58±7.12)mmHg]均较单纯染毒组明显增加,SPO2分别为(97.17±0.83)、(96.92±1.16)、(97.42±0.67)mmHg]也有所升高,差异均有统计学意义(P<0.05);33%氧浓度组和50%氧浓度组之间的差异无统计学意义(P>0.05);高压氧组PO2较另外2个氧疗组增加,差异有统计学意义(P＜0.05);高压氧组PCO2(51.42±6.60)mm Hg]较50%浓度氧组(44.58±3.42)mm Hg]增高,其他各组间PCO2的差异无统计学意义(P＞0.05).单纯染毒组大鼠天冬氨酸转氨酶(AST)(270.50±49.05)U/L]、丙氨酸转氨酶(ALT)(122.67±55.44)U/L]、尿素氮(BUN)(7.31±0.93)mmol/L]、肌酐(Cr)(28.32±4.35)μmol/L以及肌酸激酶(CK)(1808.42±582.05)U/L]和肌钙蛋白(CtnI)(22.52±14.29)ng/ml]均高于对照组,差异有统计学意义(P<0.05);而乳酸脱氢酶(LDH)(1286.58±484.80)U/L]和α-羟丁酸脱氢酶(HBDH)(553.08±53.46)U/L]无明显变化.各种方式氧疗后以上各项指标有部分降低,而高压氧治疗组AST(165.25±30.87)U/L]、HBDH(350.83±103.00)U/L]和CTnI(11.23±5.38)ng/ml]较33%浓度氧或50%浓度氧治疗组下降更为明显,差异有统计学意义(P＜0.05 ).结论 及时有效的氧疗能够明显提高急性氮气窒息大鼠动脉PO2和SPO2,并且可以改善肝功能和心肌损害.而高压氧的使用更能够明显提高对氮气窒息大鼠的治疗效果.

Abstract：

Objective To Evaluate the effects of different oxygen therapies on the rats with acute nitrogen asphyxia and to study the best oxygen therapic protocol for patients with acute nitrogen asphyxia on the spot. Methods Sixty healthy male Wistar rats were divided into 5 groups: control, exposure to nitrogen, 33% oxygen treatment, 50% oxygen treatment and hyperbaric oxygen treatment groups. The behavioral performance, arterial oxygen pressure (PO2), carbon dioxide partial pressure (PCO2) and oxygen saturation (SPO2), biochemical changes in liver and kidney function and myocardial enzymes in 5 groups were measured. Results The rats exposed to nitrogen firstly were excited then inactive symptoms, but consciousness was recovered after oxygen therapy. The PO2 and SPO2 in nitrogen exposure group were (79.67±9.12) and (94.92±2.78) mm Hg, respectively, which were significantly lower than those in control group (P<0.01). The PO2 and SPO2 of 3 oxygen treatment groups were (94.75±7.24), (94.92±8.98), (104.58±7.12)mm Hg and (97.17±0.83), (96.92±1.16), (97.42±0.67)mm Hg, respectively, which were significantly higher than those in nitrogen exposure group (P<0.05). The PO2 in hyperbaric oxygen treatment group was significantly higher than those in other 2 oxygen treatment groups (P<0.05). The SPO2 in hyperbaric oxygen treatment group was (51.42±6.60) mm Hg which was significantly higher than that (44.58±3.42)mm Hg] in 50% oxygen treatment groups (P<0.05). AST (270.50±49.05)U/L], ALT (122.67±55.44)U/L], BUN (7.31±0.93 )mmol/L], Cr(28.32±4.35) (μmol/L], CK (1808.42 ±582.05 )U/L] and CtnI (22.52±14.29 )ng/ml] in nitrogen exposure group were significantly higher than those in control group (P<0.05). AST (165.25 ±30.87 )U/L], HBDH (350.83± 103.00)U/L] and Ctnl (11.23±5.38 )ng/ml] in hyperbaric oxygen treatment group were significantly lower than those in other 2 oxygen treatment groups (P<0.05). Conclusion Timely and effective oxygen therapy can significantly increase arterial pressure of oxygen and oxygen saturation in the rats with acute nitrogen asphyxia, and can improve liver function and cardiac damage. The hyperbaric oxygen chamber can significantly increase the therapeutic effects on rats with acute nitrogen asphyxiation.

Evaluating the effects of different oxygen therapies on the rats with acute nitrogen asphyxia

Objective To Evaluate the effects of different oxygen therapies on the rats with acute nitrogen asphyxia and to study the best oxygen therapic protocol for patients with acute nitrogen asphyxia on the spot. Methods Sixty healthy male Wistar rats were divided into 5 groups: control, exposure to nitrogen, 33% oxygen treatment, 50% oxygen treatment and hyperbaric oxygen treatment groups. The behavioral performance, arterial oxygen pressure (PO2), carbon dioxide partial pressure (PCO2) and oxygen saturation (SPO2), biochemical changes in liver and kidney function and myocardial enzymes in 5 groups were measured. Results The rats exposed to nitrogen firstly were excited then inactive symptoms, but consciousness was recovered after oxygen therapy. The PO2 and SPO2 in nitrogen exposure group were (79.67±9.12) and (94.92±2.78) mm Hg, respectively, which were significantly lower than those in control group (P<0.01). The PO2 and SPO2 of 3 oxygen treatment groups were (94.75±7.24), (94.92±8.98), (104.58±7.12)mm Hg and (97.17±0.83), (96.92±1.16), (97.42±0.67)mm Hg, respectively, which were significantly higher than those in nitrogen exposure group (P<0.05). The PO2 in hyperbaric oxygen treatment group was significantly higher than those in other 2 oxygen treatment groups (P<0.05). The SPO2 in hyperbaric oxygen treatment group was (51.42±6.60) mm Hg which was significantly higher than that (44.58±3.42)mm Hg] in 50% oxygen treatment groups (P<0.05). AST (270.50±49.05)U/L], ALT (122.67±55.44)U/L], BUN (7.31±0.93 )mmol/L], Cr(28.32±4.35) (μmol/L], CK (1808.42 ±582.05 )U/L] and CtnI (22.52±14.29 )ng/ml] in nitrogen exposure group were significantly higher than those in control group (P<0.05). AST (165.25 ±30.87 )U/L], HBDH (350.83± 103.00)U/L] and Ctnl (11.23±5.38 )ng/ml] in hyperbaric oxygen treatment group were significantly lower than those in other 2 oxygen treatment groups (P<0.05). Conclusion Timely and effective oxygen therapy can significantly increase arterial pressure of oxygen and oxygen saturation in the rats with acute nitrogen asphyxia, and can improve liver function and cardiac damage. The hyperbaric oxygen chamber can significantly increase the therapeutic effects on rats with acute nitrogen asphyxiation.