钠离子对幼年期雌性大鼠骨骼生长的影响

目的 应用骨形态计量学,研究不同浓度钠离子对幼鼠骨骼生长的影响及其机制.方法 5周龄雌性SD大鼠18只,随机分为3组,分别喂饲含钠量不同的鼠粮,正常组含钠1%,高钠组含钠8%,低钠组含钠0.13%,连续喂养60 d.每天监测饮水量、食量及定期测量体重.实验结束,动物处死取左侧胫骨分成两段,进行不脱钙骨包埋,骨形态计量学...

The effect of sodium intake on bone growth in immature rats

Objective: To study the effect and mechanism of different sodium on bone growth in immature rats. Methods: Thirty-six 5-weeks-old female SD rats were randomly divided into 3 groups: low dose sodium group (LDS, 0.13% NaCl), normal dose group (NDS, 1% NaCl), and high dose sodium group (HDS, 8% NaCl). All doses of sodium were given orally by food for 60 days. The left tibia (proximal tibia metaphyses, PTM), middle (middle part of tibia shaft, TX) were taken for bone histomorphometry in the experiment. Results: In PTM, the percent trabecular bone area (BV/TV) was increased in LDS (p<0.05) and not in HDS compared with NDS group. The trabecular thickness was decreased in HDS compared with LDS group. Dynamic data showed that the bone formation rate (BFR/TV) was higher, number of osteoclast and surface perimeter of osteoclast were lower in LDS group than NDS group. No significantly different were founded between HDS group and NDS group, and No significantly different were founded between HDS group and LDS group. In Tx, There were no statistically changes in cortical static parameter in three groups. But dynamic data showed that HDS enhanced endosteal bone resorption, while LDS depressed endosteal bone resorption compared with normal group. In contrast to LDS, HDS was showed to increase percent labeling parameter and BFR in endosteal surface. Conclusion: The different effect on bone group by different concentration of sodium. Low dose sodium is benefit to bone group in immature SD female rat by increasing bone formation and decreasing bone resorption; but the effect of HDS on immature rat is vary, which may be due to individual variation.