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| WT-1通过下调c-myb表达抑制U937细胞增殖 | |
| 引用本文： | 卢青,刘革修,欧大明,曾高峰. WT-1通过下调c-myb表达抑制U937细胞增殖[J]. 中国现代医学杂志, 2000, 10(5): 8-10 |
| 作者姓名： | 卢青 刘革修 欧大明 曾高峰 |
| 作者单位： | 1. 衡阳医学院附属二医院血液内科,421001 2. 衡阳医学院药理教研室 3. 衡阳医学院附属一医院血液科 |
| 摘 要： | 研究Ｗｉｌｍｓ瘤基因在白血病发病中的作用机制。方法：采用反义、Ｎｏｒｔｈｅｒｎ ｂｌｏｔ和流式细胞技术分析了硫以义ＷＴ－１基因脱氧寡核苷酸和硫代反义ｃ－ｍｙｂ基因对Ｕ９３７细胞增殖和基因表达的影响。结果：μｍｏｌ／Ｌ ＷＴ１反义脱氧寡核苷酸处理的Ｕ９３７细胞ＷＴ１蛋白表达下降３７％，ｃ－ｍｙｂ ｍＲＮＡ水平上升不明显：１０μｍｏｌ／Ｌ ｃ－ｍｙｂ ｍＲＮＡ水平上升明显，同时Ｍｙｂ蛋白水平上升４６％ |
| 关 键 词： | U937细胞 Wilms瘤基因 脱氧寡核苷酸 白血病 |
WT1 INHIBITED PROLIFERATION OF HUMAN MONOCYTIC U937 CELLS BY DOWNREGULATING C-MYB EXPRESSINO |
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| Lu Qing,Liu Gexiu,Ou Daming,Zhen Gaofeng. WT1 INHIBITED PROLIFERATION OF HUMAN MONOCYTIC U937 CELLS BY DOWNREGULATING C-MYB EXPRESSINO[J]. China Journal of Modern Medicine, 2000, 10(5): 8-10 | |
| Authors: | Lu Qing Liu Gexiu Ou Daming Zhen Gaofeng |
| Abstract: | Objective: To elucidate a role for the Wilms tumor gene (WT1) in leukemogenesis. Methods: We investigated the effect of phosphorothioate antisense oligodeoxynucleotides (AN-S-ODNs) specific to WT1 gene on the expression of c-myb gene in U937 cells. Northern blot and flow cytometry technologies were used to detect mRNA and protein levels, respectively. Results: 5umol/L AN-S-ODNs for WT1 mRNA significantly inhibited WT1 protein expression in U937 cells at 37% (p<0.01). 10 umol/L AN-S-ODNs inhibited WT1 protein expression in U937 cells at 87% (p<0.01). And increased c-myb expression both at mRNA and protein level (46%, p<0.01), but no effect on WT1 expression in U937. Conclusions: Wilms tumor gene regulates the expression of c-myb gene expression in U937 cells. |
| Keywords: | U937 cell Wilms tumor gene c-myb gene |
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