人结肠上皮蛋白质表达谱的建立

目的:建立人正常结肠上皮的蛋白质表达谱。方法:应用双向凝胶电泳(2-DE)技术分离20例人正常结肠上皮的总蛋白质,应用基质辅助激光解吸电离飞行时间质谱(matrix-assistedlaserdesorp-tion/ionizationtimeofflightmassspectrometry,MALDI-TOF-MS)和电喷雾串联质谱(electrosprayionizationtan-demmassspectrometry,ESI-Q-TOF)鉴定其表达的蛋白质,利用生物信息学资源对所鉴定蛋白质的功能和亚细胞定位等进行分析。结果:建立了人类正常结肠上皮蛋白质的2-DE参考图谱,2-DE图谱展示了1020±50个蛋白质点,代表162种非冗余蛋白质的204个蛋白质点被鉴定,其中37种蛋白质存在翻译后修饰,并按功能和亚细胞定位对162种蛋白质进行了初步分类,这些数据可从作者的网站(http://www.xyproteomics.org)上进行查询。结论:首次建立了人正常结肠上皮的蛋白质表达谱,为研究结肠上皮的生理功能和病理过程提供了有意义的资料。

<FONT face=

OBJECTIVE: To establish a protein expression profile of human normal colonic epithelia. METHODS: Two-dimensional gel electrophoresis (2-DE) was applied to separate the total proteins of 20 human normal colonic epithelial tissues. The expression proteins in the human normal colonic epithelia were identified by both matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and electrospray ionization tandem mass spectrometry (ESI-Q-TOF), and the biological function and subcellular locations of the identified proteins were analyzed by bioinformatics. RESULTS: A 2-DE reference map of human normal colonic epithelium was established. On the 2-DE map, 1020+/-50 protein spots were detected, 204 protein spots representing 162 non-redundant proteins were identified, and 37 proteins had posttranslational modification. The identified proteins were categorized into several protein groups according to their functions or subcellular locations, whose data were available at our website (http://www.xyproteomics.org). CONCLUSION: A protein expression profile of human normal colonic epithelia is established for the first time, which provides useful information for investigating the physiological functions and pathologic process of colonic epithelia.