消毒后体温计再污染调查及整改效果评价

目的　了解消毒后体温计再污染状况 ,评价管理措施改进效果。方法　管理措施改进前后对各科室消毒后体温计及其存放容器、内垫纱布采样做细菌和真菌培养。结果　管理措施改进前共采集 137份样本 ,总污染率 5 1.82 % ,容器污染率 6 2 .5 0 % ,纱布污染率 5 2 .78% ,消毒后体温计污染率 4 1.5 1%。不同季节污染率差异无显著性 (P >0 .0 5 ) ,污染菌量差异有显著性 (P <0 .0 5 )。检出的微生物为表皮葡萄球菌、肺炎克雷伯菌、粘质沙雷菌和真菌。管理措施改进后 ,于存放第 1,2 ,4 ,6 ,7d采样培养 ,存放第 4d容器开始长菌 ,存放第 6d体温计开始长菌。结论　消毒合格的体温计如管理不善可被再污染 ,存放消毒后体温计的容器每周更换 2次 ,能有效预防再污染。

Investigation on the recontamination of thermometer after disinfection and evaluation for improvement measures

Objective To investigate on the recontamination of thermometers after disinfection and evaluate the effect of improvement of management. Methods Samples of thermometers, containers and inner-gauzes before and after the improvement of management were cultured for bacteria and fungi isolation. Results One hundred and thirty-seven samples before the improvement were taken, the total contamination rate was 51.82%, the contamination rate of containers, gauzes was 62.50% and 52.78% respectively. The contamination rate of thermometers after disinfection was 41.51%. There was no difference in contamination in different seasons (P> 0.05). The isolated bacteria were Staphylococous epidermidis, Klebsiella pneumoniae, Serratia marcescens and fungi. After improving the management measures, the bacteria began to grow on plates and thermometers after 3 days and 5 days respectively. Conclusion If thermometers were not managed properly after disinfection, they can still be contaminated. The plates storing the thermometers should be changed twice a week to prevent infection.