24例ABO血型正反定型不一致受血者的血型血清学分析

目的对ABO血型正反定型不一致问题进行血型血清学方法分析,归纳解决方案。方法对ABO正反定型不一致受血者的抗凝标本进行正反定型联合对照试验。通过对唾液中A、B、H血型物质进行测定,表明抗原存在于红细胞上,而红细胞表面的弱抗原经过吸收放散试验证明其存在,受血者ABO血型结合临床诊断予以确认。结果 1号、2号为自身溶血性贫血的2例患者;3号为B型含冷型自抗体患者;4号为A×B亚型;5号为肠道革兰阴性菌污染A型血液后产生类B抗原患者;6号为A2亚型含抗-A1患者;7号为体内含有抗-M的患者;8号为多发性骨髓瘤所致的球蛋白紊乱患者4例;9号为白血病抗体减弱患者4例;10号、11号为白血病急性发作期抗原减弱患者4例;12号、13号为2例老年患者;14号、15号为2例新生儿,因出生不满三个月,未形成同种抗体,因此,无需测定唾液A、B、H血型物质以及行吸收放散试验。结论为确保输血安全性及有效性,输血前应对血型正确鉴定。

Analysis of 24 cases of ABO blood group typing inconsistency of blood recipients of blood group serology

Objective To identify ABO blood group serological inconsistency analysis method, inductive solution. Methods Sam- ples of ABO positive and negative stereotypes of anticoagulation is not consistent in recipients of positive and negative stereotypes United controlled trial. The salivary A, B, H determination of blood group substances, suggesting that antigen present on red cells, and weak red cell surface antigen by absorption elution test to prove its existence, recipient ABO blood group according to the clinical diagnosis of confirmation.Results 2 cases of No.l, No.2 for autoimmune hemolytic anemia patients; 3 B containing cold type auto antibodies in patients with; 4 A x B subtype; No. 5 for intestinal Gram-negative bacteria pollution type A blood type B antigen from patients; 6 were A2 subtype containing anti -A1 patients; 7 as the body contains anti -M patients; 4 cases of globulin in patients with disorders of multiple myeloma in 8; 9 for leukemia antibody of 4 patients with weakened; No.lO, No.11 for acute exacerbation of leukemia antigen of 4 patients with weakened; No.12, No.13 2 cases of elderly patients; No. 14, No.15 for 2 cases of neonatal birth, because of not full three months, has not formed the same antibody, therefore, no determination of salivary A, B, H blood group substances and for absorption and elution test. Conclusion In order to ensure the safety of blood transfusion and effective, with correct identification of blood before blood transfusion.