三种胎盘间充质干细胞的分离培养及生物学特性对比研究

目的 分离培养人胎盘羊膜、绒毛膜和蜕膜三种间充质干细胞(mesenchymal stem cells,MSCs),进行生物学特性对比研究.方法 酶消化法分离胎盘羊膜、绒毛膜和蜕膜内干细胞,体外培养传代,MTT法测定细胞生长曲线,流式细胞术检测细胞表面标志,茜素红和油红O检测成骨和成脂能力.结果 表面标记鉴定分离的胎盘细胞为间充质干细胞,细胞具增殖和分化能力.三种细胞在传代过程中细胞形态、表面标志表达、分化能力维持稳定,但增殖能力存在差异.结论 成功分离获得三种胎盘MSCs,可以作为临床前研究用MSCs来源.

Isolation and biological characteristics of three kinds of human placenta-derived mesenchymal stem cells.

Objective To isolate mesenchymal stem cells from human amnions, chorions and decidua basalis, and compare their biological characteristics. Methods Stem cells were obtained by enzymatic digestion method from human amnions, chofions and decidua basalis and cultured in vitro. Cell growth curves were made by MTI method. Cell surface markers were assayed by flow cytometry. Osteogenic and adipogenic differentiation capacity was determined by alizarin red and alizarin red staining. Results Three kinds of placenta-derived mesenchymal stem cells were confirmed by cell surface markers assay. And these cells possess proliferation and differentiation ability. Cell morphology, surface markers and differentiation ability maintained stability during passage. However difference was detected in growth ability. Conclusion Three kinds of human placenta-derived stem cells were isolated and cultured successfully, and they could be used as seed cells in later preclinical study.