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Enhanced subtyping scheme for Salmonella enteritidis |
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| Authors: | Zheng Jie Keys Christine E Zhao Shaohua Meng Jianghong Brown Eric W |
| Affiliation: | University of Maryland, College Park, Maryland, USA. |
| Abstract: | To improve pulsed-field gel electrophoresis-based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the Simpson diversity index, supported this 3-enzyme combination for improved differentiation of S. Enteritidis. |
| Keywords: | Salmonella Enteritidis subtyping differentiation pulsed-field gel electrophoresis molecular epidemiology clone restriction endonuclease genetic diversity dispatch |
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