MKK-4、MMP-9基因的表达与原发性肝癌肿瘤侵袭转移的关系

目的:检测m itogen activated prote in k inase k inase 4(MKK-4)、MMP-9基因在原发性肝癌中的表达,探讨原发性肝癌MKK-4与MMP-9 mRNA表达水平间的相互关系,及两者对原发性肝癌侵袭转移的影响。方法:应用逆转录-聚合酶链反应(RT-PCR)检测34例原发性肝癌癌组织和相应癌旁组织及12例正常肝组织中MKK-4 mRNA、MMP-9 mRNA的表达。结果:癌旁与正常肝组织的MMP-9 mRNA表达差异无显著统计学意义(P>0.05),癌中的MMP-9 mRNA表达增高,且转移癌与未转移癌组间有差异(P<0.05),转移癌组、未转移癌组分别与正常组及癌旁组织比较有差异(P<0.01);MKK-4 mRNA在正常及癌旁组织中表达无差异(P>0.05),转移癌组、未转移癌组分别与正常组及癌旁组比较有差异(P<0.01),转移癌中较未转移癌中的表达低(P<0.05);MKK-4或MMP-9的mRNA表达均与肿瘤的大小、分化无关(P>0.05);MKK-4 mRNA与MMP-9 mRNA的表达有一定的相关性(r=-0.925,P<0.01)。结论:提示MKK-4 mRNA与MMP-9 mRNA的表达改变与原发性肝癌侵袭转移的发生发展有一定的关系。

Relationship between expression of MKK-4 and MMP-9 genes in primary hepatic carcinoma with or without invasion and metastasis

AIM: To investigate the expression of mitogen activated protein kinase kinase 4(MKK-4) and MMP-9 mRNA in primary hepatic carcinoma(PHC),and to analyze its relationship with invasion and metastasis.METHODS: The expression of MKK-4 and MMP-9 mRNA in 34 cases of hepatic carcinoma tissues and adjacent tissues,and in 12 cases of normal liver tissues were detected with RT-PCR.RESULTS:(1) The expression level of MMP-9 mRNA was higher in metastatic cancer tissues than that in other tissuses(P<0.01).(2) There was significant statistical difference among the expression level of MKK-4 mRNA,but the level in metastatic cancer was low(P<0.01).(3) There was no statistical difference among the expression level of MKK-4 or MMP-9 mRNA among the adjacent tissues and normal tissues(P>0.05).(4) MMP-9 mRNA had a tendency to rise as PHC became invasive and metastatic.The expression level of MKK-4 had a tendency to decline in PHC became invasive and metastatic.(5) The expression level of MMP-9 or MKK-4 mRNA had no correlations with tumor volume,or cell differentiation(P>0.05).(6) There were correlations between expressions of MKK-4 and MMP-9 mRNA in PHC(Pearson Correlation,r=(-0.925),P<0.01).CONCLUSION: There are high MMP-9 mRNA expression and low MKK-4 mRNA expression in PHC.The expression level of MKK-4 or MMP-9 mRNA is correlated with tumor metastasis.