人脐静脉内皮细胞表达CD137分子及其功能的初步研究

目的　了解人脐静脉内皮细胞 (HUVEC)CD137分子的表达情况 ,探讨其对内皮细胞功能的影响。方法　胰蛋白酶法分离培养人脐静脉内皮细胞 ,用RT PCR、定量荧光PCR和流式细胞仪技术 ,分别观测未刺激和用内毒素 (LPS)刺激的内皮细胞CD137mRNA与蛋白的表达情况。用anti CD137单克隆抗体交联内皮细胞表面CD137,ELISA检测其上清液中IL 8的含量。结果　①RT PCR和实时荧光定量RT PCR结果显示 ,未经刺激的人脐静脉内皮细胞表达少量CD137mRNA ,流式细胞仪检测证实其细胞表面表达少量CD137蛋白 ;当用LPS刺激内皮细胞培养 2 4h后 ,mRNA和蛋白表达量均显著提高。②ELISA检测发现 ,anti CD137单克隆抗体交联内皮细胞表面CD137分子后 ,和同型抗体对照组相比 ,其培养上清液中细胞因子IL 8的表达量明显提高 ,与LPS刺激内皮细胞上调IL 8表达的程度相当。结论　人脐静脉内皮细胞组成性表达少量CD137分子 ,LPS可以显著上调其表达。交联内皮细胞表面CD137分子可提高其IL 8产生和分泌 ,提示其具有刺激内皮细胞活化的生物功能

Studies of the expression of CD137 in human umbilical vein endothelial cells and its function

Objective To detect the expression of human CD137 in human umbilical vein endothelial cells (HUVECs) and its function. Methods The expression of human CD137 on HUVECs was detected by semi-quantitative RT-PCR and fluorescence-activated cell sor- ting (FACS). The level of IL-8 was detected with ELISA. Results The expressions of CD137 were detected in LPS-stimulated and unstimula- ted HUVECs. The IL-8 secretion was increased by cross-linking of CD137 mAbs and LPS stimulation. Conclusion HUVEC can constitutively express CD137, and LPS stimulation can enhance this expression. Both LPS and CD137 can increase the IL-8 secretion of HUVEC.