丹参红花提取物对鼠心脏成纤维细胞胶原合成的影响

目的:探讨丹参红花提取物(DCE)对血管升压素(AVP)诱导的新生SD大鼠心脏成纤维细胞(CF)胶原合成作用的影响,为应用中成药防治高血压心肌纤维化提供理论依据.方法:运用3H-脯氨酸掺入法分别观察不同浓度和不同时间DCE对AVP诱导CF胶原合成的影响.结果:CF的3H-脯氨酸掺入率随着DCE干预浓度的增加而降低,其中100mg/L和200mg/L DCE组的3H-脯氨酸掺入率明显低于空白对照组,并具有统计学意义(P<0.01);随着DCE作用时间的延长,CF的3H-脯氨酸掺入率呈递减趋势,CF胶原合成功能与DCE作用时间呈明显的负相关.结论:DCE能够呈浓度和时间依赖性抑制AVP诱导的CF胶原合成功能,防治高血压心肌纤维化.

Effects of Danshen root and Carthamus tinctorius Extract on the collagen synthesis of rat cardiac fibroblasts

To investigate the effects of Danshen root and Carthamus tinctorius Extract(DCE) on the collagen synthesis of rat cardiac fibroblasts (CF).Methods:CF of neonatal Sprague-Dawley (SD) rats were isolated by trypsin digestion method and growth-arrested CF were stimulated with 10^-7mol/L AVP in the presence of varying concentrations of DCE(10mg/L,50mg/L,100mg/L and 200mg/L).Collagen synthesis was measured by ³H-proline incorporation.Results:The results showed that:DCE decreased ³H-thymidine incorporation in CF with a concentration dependent manner.³H-thymidine incorporation values of 100mg/L and 200mg/L DCE groups are 502±35.13cpm/2000cells and 408±24.58cpm/2000cells, respectively, which are significantly lower than that of the control (both P<0.01);With the increase of time, ³H-thymidine incorporation of CF decrease gradually, there are obviously negative relevance between ³H-thymidine incorporation and time.Conclusion:The results indicate that DCE decreases CF collagen synthesis.