MicroRNA-145在急性髓系白血病患者中的表达及意义

目的：探讨MicroRNA‐145（miR‐145）在急性髓系白血病患者骨髓细胞中的表达情况，并分析其临床意义。方法收集急性髓系白血病患者骨髓标本70例，其中初治组46例、缓解组24例，非血液肿瘤性疾病14例作为对照组。通过实时荧光定量PCR方法检测miR‐145的相对表达水平。结果 miR‐145在初治急性髓系白血病骨髓中表达水平明显低于缓解组及对照组（P＜0．01）；其中9例经化疗后缓解的急性髓系白血病患者骨髓miR‐145表达水平较治疗前显著升高，差异有统计学意义（P＜0．05）；且miR‐145表达水平与初诊时原始细胞百分数及外周血白细胞数均无相关性（P＝0．456、0．394）。结论 miR‐145在急性髓系白血病患者表达下调，提示miR‐145的表达与白血病的发生、发展存在显著的关系。

Expression and significance of miR-145 in acute myeloid leukemia

Objective To research the expression and clinical significance of miR‐145 in the marrow of acute myeloid leuke‐mia (AML) .Methods A total of 70 cases of bone marrow samples were collected from AML patients ,of which included 46 cases of new diagnosed patients(initial treatment group) ,24 cases of AML patients in complete remission(remission group) .Fourteen ca‐ses of non‐neoplastic patients were used as controls(control group) .The miR‐145 relative expression level was detected by real‐time quantitative PCR .Results The relative expression level of miR‐145 in initial treatment group was less than those of control group and remission group(P<0 .01) .Compared to before‐chemotherapy patients ,the expression level of miR‐145 in the 9 case of AML patients with complete remission after chemotherapy increased significantly(P<0 .05) .The relative expression of miR‐145 showed no significantly correlation with the percentage of blasts cells in bone marrow and the percentage of peripheral blood leukocyte count (P=0 .456 ,0 .394) .Conclusion The expression of miR‐145 in bone marrow cells of AML patients is down‐regulated ,which sug‐gested the development of acute leukemia .