辛伐他汀对高糖培养肾小球系膜细胞增殖和细胞周期的影响及机制

目的：研究辛伐他汀（SIM ）对高糖培养肾小球系膜细胞（GMC）增殖和细胞周期的影响，并观察其炎症因子和细胞外基质的变化。方法将大鼠 GMCs 分为4组：低糖组[LG 组，葡萄糖（Glu）5．6 mmol／L ]，高糖组（HG 组，Glu 30 mmol／L ）， HG ＋ SIM （10μg／L）组和 HG ＋ SIM （25μg／L）组。采用4-甲基偶氮四唑蓝（M TT ）法检测各组细胞的增殖能力，同时比较各组细胞的 G0／G1，G2＋ M 期和 S 期细胞比例。比较各组细胞上清液中的转化生长因子β1（TGF-β1）、纤维黏连蛋白（FN）和Ⅳ型胶原（COL4）水平。结果 HG 组不同时刻的吸光度（A）高于 HG ＋ SIM （10μg／L）组、HG ＋ SIM （25μg／L）和 LG 组，差异有统计学意义（P＜0．05）。 LG 组的 G0／G1期细胞比例为（35．67±3．38）％，S 期细胞比例为（41．24±5．64）％；HG 组的 G0／G1期细胞比例为（25．88±4．02）％，S 期细胞比例为（28．65±1．88）％；HG ＋ SIM （10μg／L）组的 G0／G1期细胞比例为（28．12±2．01）％，S 期细胞比例为（35．55±2．09）％；HG ＋ SIM （25μg／L）组的 G0／G1期细胞比例为（31．85±3．52）％，S 期细胞比例为（39．82±2．23）％，差异有统计学意义（P＜0．05）。 HG 组上清液 TGF-β1、FN 和 COL4水平高于 HG ＋ SIM （10μg／L）组、HG ＋ SIM （25μg／L）和 LG组，差异有统计学意义（P＜0．05）。结论 SIM 可以通过降低 TGF-β1水平来抑制 GMCs 的增殖并调整细胞周期，抑制 GMCs 肥大。

Effect and mechanism of simvastatin on proliferation and cycle of glomerular mesangial cells cultured in high-concentration glucose

Objective To study the effect of simvastatin(SIM ) on the proliferation and cycle of glomerular mesangial cells (GMCs) cultured in high concentration of glucose ,and to observe the variation of inflammation factor and extracellular matrix . Methods GMCs of rats were divided into 4 groups :low glucose(LG) group(Glu 5 .6 mmol/L) ,High glucose (HG) group(Glu 30 mmol/L) ,HG + SIM (10 μg/L)group and HG + SIM group(25 μg/L) .Cells′ proliferation activity was tested by M TT ,simultane-ously compared the ratio of cells in different period (G0 /G1 ,G2 + M and M ) ,and levels of TGF-β1 ,FN and COL4 were compared a-mong different groups .Results Absorbancy (A) of different periods in HG group was higher than that in other 3 groups ,difference had statistical significance(P< 0 .05) .In LG group ,the ratio of cells in G0 /G1 were(35 .67 ± 3 .38)% while S-stage were(41 .24 ± 5 .64)% ;in HG group ,the ratio of cells in G0 /G1 were(25 .88 ± 4 .02)% while S-stage were(28 .65 ± 1 .88)% ;in HG + SIM (10 μg/L)group ,the ratio of cells in G0 /G1 were(28 .12 ± 2 .01)% while S-stage cell were(35 .55 ± 2 .09)% ;in HG + SIM (25 μg/L ) group ,the ratio of cells in G0 /G1 were(31 .85 ± 3 .52)% ,while in S-stage were(39 .82 ± 2 .23)% ,the difference had statistical sig-nificance(P< 0 .05) .Levels of TGF-β1 ,FN and COL4 in HG group were higher than those of in HG + SIM (10 μg/L)group ,HG +SIM (25 μg/L)group and LG group ,the difference had statistical significance(P< 0 .05) .Conclusion By decreasing TGF-β1 level , simvastatin can inhibit the proliferation of GMCs and regulate the cell cycle ,to suppress the hypertrophy of GMCs .