通过Micro-CT评价瘦素受体缺乏的2型糖尿病小鼠四肢骨的骨代谢变化

目的 探讨瘦素受体(leptin receptor,Lepr)缺乏的2型糖尿病小鼠的骨骼表型,为2型糖尿病((T2DM)合并骨质疏松(OP)的防治提供一个新的靶点.方法 获取20只14周龄雄性db/db小鼠(瘦素受体缺乏小鼠)和野生型小鼠(C57BL小鼠)的胫骨(各10只),通过Micro-CT检测比较两者骨小梁相对体...

The changes of bone metabolism in the extremities of type 2 diabetic mice with leptin receptor deficiency were evaluated by micro-CT

Objective To investigate the skeletal phenotype of type 2 diabetic mice with Leptin receptor (Lepr) deficiency and to provide a new target for the prevention and treatment of type 2 diabetes mellitus (T2DM) combined with osteoporosis (OP). Methods Get 20 males only 14 weeks of db/db mice (lack of leptin receptor) in mice and wild-type mice (C57BL mice) shin (10), through the Micro-CT detection to compare both trabecular bone volume (BV/TV) relatively, the surface area of bone tissue volume ratio (BS/TV), bone trabecular thickness (Tb.Th), bone trabecular number (Tb. N), separating degree (Tb. Sp), trabecular bone bone structure model index (SMI), bone cortical thickness (Ct.Th), bone cortex area (Ct.Ar) bone microstructure parameters, such as difference. Results Compared with wild type mice, 14 weeks of db/db mice tibial trabecular bone volume (BV/TV) and bone trabecular thickness (Tb. Th), bone trabecular number (Tb. N) significantly reduced, trabecular bone spacing (Tb. Sp) increased, the corresponding cortical thickness (Ct.Th), cross-sectional area (Ct.Ar) decreased, and compared the differences between all had significant statistical significance (P < 0.05);The structure model index (SMI) was significantly lower than that of the wild type, and the difference between the two was statistically significant (P < 0.05). Conclusion Type 2 diabetes may affect the change of bone mass through the signal pathway involved in leptin receptor, which provides a new direction for the study of DOP etiology and treatment methods by using this model. In addition, in the absence of leptin signal transduction, bone mass and strength decrease, which proves that leptin plays a role of anabolic bone factor in the body.