淫羊藿治疗糖皮质激素性骨质疏松症分子机制的网络药理学分析

目的 利用网络药理学方法探索淫羊藿治疗糖皮质激素性骨质疏松症(glucocorticoid-induced osteoporosis,GIOP)的分子作用机制.方法 运用中药系统药理学数据库与分析平台(TCMSP)筛选淫羊藿的活性成分及相关靶点;运用GeneCards数据库、OMIM数据库获取GIOP的疾病靶点;通过R...

Network pharmacology analysis of the molecular mechanism of herba epimedii in the treatment of glucocorticoid-induced osteoporosis

Objective To explore the molecular mechanism of herba epimedii in the treatment of glucocorticoid-induced osteoporosis (GIOP) with network pharmacology. Methods The active ingredients and related targets of herba epimedii were screened with Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The disease target of GIOP was obtained with Used GeneCards database and OMIM database. The drug target was intersected with the GIOP disease target through R language. The Cytoscape 3.7.0 software was used to construct a network of herba epimedii-active ingredient-GIOP target. Herba epimedii-GIOP protein-protein interaction (PPI) network was constructed using the String database. The gene ontology (GO) biological function enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway were analyzed using R language. Results Twenty-three major active ingredient of herba epimedii were obtained through the screening, and a total of 65 GIOP-related targets were involved. Quercetin, kaempferol, luteolin, and icariin connected with more than 10 related targets, which were the main components of epimedium in the treatment of GIOP. IL-6, VEGFA, MAPK8, CASP3, and ESR1 were the five most frequently linked targets in the interaction of common target protein of epimedium-GIOP, which were the core target genes. Conclusion Herba epimedii may have the treatment effect on GIOP through prostate cancer pathway, cell apoptosis pathway, fluid sheer stress and atherosclerosis pathway, TNF signal pathway, and AGE-RAGE signal pathway in diabetes. This provides a new thought and direction for the study of the mechanism of pharmacodynamics.