当归药材及当归身饮片高效液相色谱特征图谱研究

目的:建立当归药材及当归身饮片高效液相色谱(HPLC)特征图谱,并通过对当归药材和当归身饮片特征图谱的比较分析,为当归药材和当归身饮片的质量控制提供参考依据.方法:采用Symmetry C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.2％乙酸为流动相,梯度洗脱,检测波长为280 nm,柱温为30℃,流速...

Research on HPLC Specific Chromatogram of Angelica sinensis and Angelica Body Decoction Pieces

Objective:To establish the HPLC specific chromatogram of Angelica sinensis and Angelica sinensis body,and to provide reference for the quality control of Angelica sinensis and Angelica body by the comparative analysis of the characteristic maps of Angelica sinensis and Angelica body.Methods:The HPLC analysis was performed on an Symmetry C₁₈ column(250 mm×4.6 mm,5μm)with acetonitrile-0.2%acetic acid solution as the mobile phase with gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was 280 nm.The column temperature was 30℃.The flow rate was 1.0 mL/min,and the injection volume was 20μL;Similarity evaluation system of chromatographic fingerprint of Chinese medicine was used to evaluate the similarity of 15 batches of Angelica sinensis and Angelica body in 3 production areas,and to establish the characterisics fingerprint of Angelica sinensis and Angelica body.Results:The specific chromatogram was preliminarily constructed and 4 common peaks of ferulic acid,senkyunolide I,senkyunolide H and ligustilide with ferulic acid as the reference were identified.The similarity of 15 batches of Angelica sinensis was greater than 0.96,and the similarity of 15 batches of angelica body was greater than 0.97.The characteristics of the HPLC specific chromatogram of Angelica sinensis and angelica body were similar,but the content of each component was different.Conclusion:The method is stable,reliable and reproducible,and can be used for quality control of Angelica sinensis and angelica body,and provide a basis for the development of famous classical formulas containing Angelica sinensis or angelica body.