动脉粥样硬化高脂饲料对小鼠糖脂水平的作用研究

目的 研究动脉粥样硬化高脂饲料对小鼠体重、血糖血脂水平及动脉粥样硬化斑块形成的作用,并且明确禁食时间对血脂检测结果的影响.方法 对10周龄雄性ApoE基因敲除(ApoE-/-)小鼠分别给予高脂饲料及普通饲料,4个月后观察动脉粥样硬化斑块情况;对10周龄雄性C57BL/6J小鼠分别给予普通饲料4周、普通饲料2周+高脂饲料...

Effect of atherosclerotic high-fat diet on the level of glucose and lipid in mice

Objective To study the effects of atherosclerotic high-fat diet on body weight,blood glucose,blood lipid levels and atherosclerotic plaque formation in mice,and to determine the effect of fasting time on the results of blood lipid testing.Methods 10-week-old male ApoE knockout(ApoE-/-)mice were given high-fat diet and normal diet.The atherosclerotic plaques were observed four months later.10 week old C57 BL/6 J male mice were given regular diet for 4 weeks,regular diet for 2 weeks+high-fat diet for two or four weeks.Body weight、liver、glucose,and the serum lipid levels were examined.The influence of fasting for 12 h,6 h or no fasting on blood lipid detection results before sacrificing were studied.Results The atherosclerotic plaque area of ApoE-/-mice given high-fat diet increased significantly(P<0.01).C57 BL/6 J mice given high-fat diet gained weight(P<0.01).The glucose,TC,LDL-c and HDL-c were also increased in C57 BL/6 J mice with liver fat accumulation while the level of TG was significantly decreased(P<0.01).Compared with the fasting 12 h group,serum triglyceride(TG)was significantly increased(P<0.01)in fasting 6 h and no fasting groups.Conclusion The atherosclerotic high-fat diet can accelerate the formation of atherosclerotic plaques in ApoE-/-mice,significantly increase blood sugar,TC and LDL-c levels,but significantly reduce TG values..The fasting time can affect serum triglyceride(TG)level.