澳洲茄胺对人骨肉瘤移植瘤的抑制作用及其机制

目的 探讨澳洲茄胺对人骨肉瘤移植瘤的抑制作用及其机制.方法 该研究于2018年1月至2019年3月完成.MG63细胞株购于中科院上海细胞库.将0.2 mL人骨肉瘤MG63细胞悬液(共2×106个细胞)注射于裸鼠右侧腋窝皮下,建立人骨肉瘤移植瘤模型,实验分为4组,即对照组和澳洲茄胺组(10 mg/kg、20 mg/kg、...

Inhibitory effect of solasodine on human osteosarcoma xenografts and its mechanism

Objective To explore the inhibitory effect of solasodine on human osteosarcoma xenografts and its mechanism.Meth? ods The study was completed from January 2018 to March 2019. The MG63 cell line was purchased from Shanghai Cell Bank of Chinese Academy of Sciences. The human osteosarcoma MG63 cell suspension (0.2 mL, 2×106 cells) was injected subcutaneously into theright armpit of nude mice to establish human osteosarcoma xenograft model. The experiment was assigned into four groups: controlgroup and solasodine group (10 mg/kg, 20 mg/kg, 40 mg/kg). The mice were injected intraperitoneally once every other day for 4 weeks.The weight and volume of xenografts were measured. Apoptosis was detected by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining. The expression of apoptosis-related proteins, angiogenesis-related factors and phosphoinositide-3-kinase/serine-threonine kinase (PI3K/AKT) signaling pathway were detected by Western blotting.Results Compared with control group, the weight (0.45±0.12) g and volume (510.62±95.78) mm3 at 21 d and (579.56±92.04) mm3 at 28 d of the xenografts in the solasodine group decreased, and the inhibitory effect of 40 mg/kg group was the most obvious (P<0.05). The fluorescence intensity of terminal-deoxynucleotidyl transferase mediated nick end labeling-fluorescein-isothiocyanate (TUNEL-FITC) in the solasodine group was higher thanthat in the control group, especially in the 40 mg/kg group. The protein expression of B cell lymphoma/leukemia-2 associated X protein (Bax) (3.55±0.39), cleaved cystein-asparate protease-3 (Cleaved caspase-3) (3.94±0.33), cleaved cystein-asparate protease-9 (Cleaved caspase-9) (3.59±0.31), thrombospondin 1 (TSP-1) (3.70±0.41) were up-regulated, while the protein expression of B cell lymphoma/leukemia-2 (Bcl-2) (0.31±0.15), vascular endothelial growth factor (VEGF) (0.25±0.12), phosphorylated phosphoinositide-3-kinase (p-PI3K) (0.39±0.12) and phosphorylated serine-threonine kinase (p-AKT) (0.33±0.11) were down-regulated, and the effect of 40 mg/kg group was the most obvious (P<0.05).Conclusion Solasodine can inhibit the growth of human osteosarcoma cells, induce apoptosis, down-regulate the expression of VEGF and up-regulate the expression of TSP-1, which may be related to the inhibition of PI3K/AKT signaling pathway.