当归汤多糖对溃疡性结肠炎的改善作用及机制初步研究

[目的]探讨当归汤多糖改善溃疡性结肠炎(UC)的作用和机制.[方法]构建2,4,6-三硝基苯磺酸(TNBS)诱导的UC大鼠模型,通过疾病活动指数(DAI)、结直肠宏观损伤评分、苏木精-伊红(HE)染色的结直肠病理评分,评价当归汤多糖对UC的干预作用,并检测结直肠组织氧化应激指标[超氧化物歧化酶(SOD)、丙二醛(MDA...

Preliminary study on the improvement effect and mechanism of Danggui Decoction polysaccharide eextracts on ulcerative colitis

[Objective]To explore the effect and mechanism of Danggui Decoction polysaccharide extracts(DGDPE)in improving ulcerative colitis(UC).[Methods]AUC rat model induced by 2,4,6-Trinitrobenzenesulfonic acid(TNBS)was established to evaluate the effects of DGDPE on UCthrough disease activity index(DAI),colorectal macroscopic injury score,and HE staining colorectal pathology score.RAW264.7 cell model was used to detect the influence of DGDPE on NO production.Spleen cells of normal and UC rats were taken to study the effect of DGDPE on the proliferation of spleen cells under the stimulation with Con-A,and study on mechanism by detecting colorectal tissue oxidative indicators(SOD,MDA,MPO),IL-2 and IFN-γ levels,andspleen cell proliferation.[Results]Rats in the model group had mucus and bloody stools,and the above indicators were significantly different from those in the control group.Compared with the model group,the DAI index,macroscopic injury score,colorectal pathology score,spleen cell stimulation index,IL-2 and IFN-γ levels,MDA content,and MPO activity significantly decreased,and SOD activity significantly increased.Compared with the blank group,DGDPE could significantly increase the production of NO in RAW264.7 cells.But the NO levels of all DGDPE treated groups were significantly lower than that of the positive control stimulated by 1μg/mL LPS.Compared with the model group,DGDPE could significantly promote the proliferation of normal rat spleen cells and inhibit the proliferation of UC rat spleen cells under the stimulation with Con-A.[Conclusion]DGDPE has an improvement effect on TNBS-induced UC in rats,and its mechanism may be related to the regulation of immunity inhibition and alleviation of oxidative stress.