运动对高脂饲养大鼠白色脂肪组织血管内皮生成相关因子的影响

目的:观察8周中等强度有氧运动干预以及高脂饮食对雄性大鼠血管内皮生成相关因子的影响.方法:40只3周龄SPF级雄性SD大鼠适应性喂养3天后,随机分为标准安静组(CS)、标准运动组(CE)、高脂安静组(HS)、高脂运动组(HE).标准组大鼠喂饲普通饲料(D12450B),高脂组大鼠喂饲高脂饲料(D12451),所有大鼠均...

Effects of exercise on vascular endothelial growth related factors in white adipose tissue of high-fat fed rats

Objective:To observe the effects of 8-week moderate-intensity aerobic exercise and high-fat diet on vascular endothelial growth-related factors of male rats.Method:After adjustable fed for 3 days,forty three-week-old SPF male SD rats were randomly divided into standard quiet group(CS),standard exercise group(CE),high-fat quiet group(HS),and high-fat exercise group(HE).Rats in the standard group were fed with normal diet feed(D12450B),while high-fat diet feed(D12451)in the high-fat group.All rats were free to eat and drink,and rats in the CE and HE groups underwent exercise intervention for 8 weeks(60%—70%VO2max,1h/time,5 times/week,each training includes 5min warm-up,50min treadmill training,5min recovery).All materials were taken after 8 weeks of intervention(inguinal adipose tissue,epididymal white adipose tissue and perirenal white adipose tissue).The relative expression levels of VEGF,VEGFR2,and CD31 mRNA were measured by RT-qPCR.Western Blot was used to detect the expression of uncoupling protein 1(UCP-1)in inguinal adipose tissue and epididymal white adipose tissue.Result:①Compared with the CS group,the relative expressions of VEGFR2 and CD31 mRNA in the inguinal adipose tissue of the HS group were significantly reduced(P<0.05),and the UCP-1 protein expression was also significantly reduced(P<0.01).The relative expression of CD31 mRNA in epididymal white adipose tissue were significantly increased(P<0.01),the expression level of UCP-1 protein was significantly reduced(P<0.01).The relative expressions of VEGF,VEGFR2,CD31 mRNA in perirenal white adipose tissue were significantly increased(P<0.01).②Compared with the HS group,the relative expression of VEGFR2 mRNA in the inguinal adipose tissue of the HE group was significantly increased(P<0.01),the protein expression of UCP-1 was significantly reduced(P<0.01).The relative expressions of VEGF,VEGFR2,and CD31 mRNA in tepididymal white adipose tissue were significantly reduced(P<0.01),and UCP-1 protein expression was also significantly reduced(P<0.01).The relative expression levels of VEGF,VEGFR2,and CD31 mRNA in the perirenal white adipose tissue were significantly increased(P<0.01).Conclusion:The effects of 8-week high-fat diet and exercise on the mRNA expression levels of angiogenesis related factors in different white adipose tissue of rats were different,and the mechanism of action remained to be further explored.