| 重组P_(53)真核表达载体的构建 |
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| 引用本文： | 李仁 左祖英. 重组P_(53)真核表达载体的构建[J]. 川北医学院学报, 1998, 13(1): 1-4 |
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| 作者姓名： | 李仁 左祖英 |
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| 作者单位： | 川北医学院分子生物学研究室 |
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| 摘 要： | 为进一步探索Ｐ５３基因与机体发育、肿瘤发生的关系及其与细胞周期中其它调控因子的相互作用，我们采用粘性末端连接法构建了重组野生型Ｐ５３－ＰＸＴ１真核表达载体，并成功地转化Ｃａｃｌ２处理的ＲＲ １细胞，经多种方法鉴定表明其连接率为６９％，转化率为３９×１０３／μｇＤＮＡ。该重组载体的构建无疑将为肿瘤、胎儿畸形及着色性干皮病等的病因及诊治研究提供物质基础
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| 关 键 词： | 重组 P_((53)基因 表达载体 构建 |
Construction of Recombinant P 53 Eukaryotic Expression Vector |
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| Li Ren et al. Construction of Recombinant P 53 Eukaryotic Expression Vector[J]. Journal of North Sichuan Medical College, 1998, 13(1): 1-4 |
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| Authors: | Li Ren et al |
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| Affiliation: | Li Ren et al Department of Molecular Biological Laboratory |
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| Abstract: | To further investigate the relation of P 53 gene to mammals development and tumour occurrence,as well as the effects of P 53 gene in cell cycle,We constructed recombinant wild type P 53 ～PXT 1 expression vector.The screening result of the recommants with several methods suggestted the ligation rate was 69%,its transformation rate was 3.9×10 3/μgDAN.Constructing recombinant P 53 vector will be likely to provide new ideals,new methods and targete moleculcor for diagnosis and therapy of developmental abnormalities,tumour and other diseases. |
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| Keywords: | Recombinant P 53 gene Expression vector Construction |
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