| 抗三尖杉酯碱的HL60细胞抗粉防己碱诱导的细胞凋亡 |
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| 引用本文： | 何琪杨,张鸿卿,庞大本,池旭生,薛绍白.抗三尖杉酯碱的HL60细胞抗粉防己碱诱导的细胞凋亡[J].中国药理学报(英文版),1996(6). |
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| 作者姓名： | 何琪杨 张鸿卿 庞大本 池旭生 薛绍白 |
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| 作者单位： | 北京师范大学生物系，中国中医研究院基础理论研究所 |
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| 基金项目： | the National Natural Science Foundation of China,The Doctoral Programme Foundation for Institution of Higher Education |
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| 摘 要： | 抗三尖杉酯碱的ＨＬ６０细胞抗粉防己碱诱导的细胞凋亡１何琪杨，张鸿卿，庞大本２，池旭生２，薛绍白３（北京师范大学生物系，北京１００８７５；２中国中医研究院基础理论研究所，北京１００７００，中国）目的：用粉防己碱（Ｔｅｔ）研究抗三尖杉酯碱（Ｈａｒ）的ＨＬ...
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| 关 键 词： | 细胞凋亡 粉防己碱 三尖杉酯碱类 白血病HL60 c－myc基因 抗药性 磷酸化 |
Resistance to apoptosis of harringtonine resistant HL60 cells
induced by tetrandrine 1 |
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| HE Qi Yang,ZHANG Hong Qing,PANG Da
Ben ,CHI Xu Sheng ,XUE Shao Bai.Resistance to apoptosis of harringtonine resistant HL60 cells
induced by tetrandrine 1[J].Acta Pharmacologica Sinica,1996(6). |
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| Authors: | HE Qi Yang ZHANG Hong Qing PANG Da
Ben CHI Xu Sheng XUE Shao Bai |
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| Institution: | HE Qi Yang,ZHANG Hong Qing,PANG Da
Ben 2,CHI Xu Sheng 2,XUE Shao Bai 3 |
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| Abstract: | IM: To study the
mechanism of resistance to apoptosis in the harringtonine (Har)
resistant HL60 cells with tetrandrine (Tet). METHODS: Growth
inhibition, flow cytometry, DNA agarose gel electrophoresis, protein
phosphorylation, and RNA dot hybridization. RESULTS: The resistant
cells had no cross resistance to Tet. Tet induced the sensitive but
not the Har resistant HL60 cells to apoptosis. The high
phosphorylation of protein <30 kDa occurred when the resistant
cells were treated with Tet. Tet and Har increased the expression of
c myc mRNA in the sensitive HL60 cells. The expression of c
myc mRNA in the resistant cells was obviously decreased and almost
not changed in treatment with Tet and Har. CONCLUSION: Tet induced
the sensitive but not the Har resistant HL60 cells to apoptosis, and
the resistance to apoptosis induced by Tet was associated with the
high protein phosphorylation and reduction of the expression of c
myc mRNA. |
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