通心络对大鼠脑缺血再灌注模型基质金属蛋白酶-9的影响

目的通过比较大鼠脑缺血再灌注损伤中基质金属蛋白酶- 9(MMP -9)的表达,研究中药制剂通心络对缺血脑组织的作用机制。方法雄性Wistar大鼠,随机分为24 h对照组、24 h通心络组、5 d对照组、5 d通心络组以及假手术组。线栓法建立大脑中动脉阻塞90 min的脑缺血再灌注模型。通过免疫组织化学染色方法测定MMP- 9的表达。结果通心络治疗后24 h及5 d通心络组缺血侧MMP 9的表达较对照组下降(P< 0 .05)。结论通心络可能通过抑制MMP- 9 的表达起到减轻大鼠脑缺血再灌注损伤的作用。

Effect of Tongxinluo on expression of matrix metalloproteinase-9 in a rat model of transient middle cerebral artery occlusion

ObjectiveTo investigate the effect of Tongxinluo by the dynamic changes of the expression of matrix metalloproteinase-9(MMP-9) on local cerebral ischemic-reperfusion injury in rats.MethodsMale Wistar rats were randomly divided into sham-operated control group, normal saline control groups (24-hour and 5-day) and Tongxinluo treatment groups(24-hour and 5-day). Animals in the latter four groups were subjected to focal ischemia by filament occlusion of the middle cerebral artery for 90 min. At the different end point, the expression of MMP-9 were assayed using immunohistochemistry staining method. ResultsCompared with the control groups, the expressions of MMP-9 were significantly decreased in both 24-hour and 5-day Tongxinluo treatment groups(P<0.05).ConclusionThe protective effects of Tongxinluo on cerebral ischemia reperfusion injury may be mediated by pharmacological inhibition of MMP-9 activity.The protective effects of Tongxinluo on cerebral ischemia reperfusion injury may be mediated by pharmacological inhibition of MMP-9 activity.