褪黑激素对老年大鼠肝MDA含量和GSH—px、CAT，Ca^2＋—ATPase活性的影响

为探讨褪黑激素保肝抗衰老的作用及其机理 ,文章观察了褪黑激素对老年大鼠肝过氧化脂质产物含量和氧自由基、细胞内钙离子清除能力的影响。连续 30天给初老大鼠补充褪黑激素后 ,用生化比色法测定了肝组织内丙二醛 (MDA)含量和谷胱甘肽过氧化物酶 (GSH- px) )、氢过氧化物酶(CAT)、钙离子 -三磷酸腺苷酶 (Ca2 - ATPase)活性。与对照组比较 ,实验组大鼠肝内 MDA含量下降了 31.2 % ,GSH- px、CAT和 Ca2 - ATPase活性分别提高了 2 5.1%、49.6%和 2 5.4%。提示 ,褪黑激素能增强肝细胞抗氧化能力和清除细胞内游离钙离子的能力 ,具有一定的保护肝细胞和抗衰老作用。

Effect of Melatonin on Content of MDA and Activities of GSH px,CAT,Ca~(2 )-ATPase In The Aging Rat Liver

For exploration the anti aging action of melatonin in liver, the effect of melatonin on the production of lipid peroxide and the activities of oxygen free radical clearing system and calcium clearing system in the aging rat liver was studied. Melatonin was used to replenish to newly old rats for 30 days. The content of malonaldehyde(MDA) and the activities of glutathione peroxidase(GSH px),catalase(CAT),Ca 2 adensine triphosphatase(Ca 2 ATPase) were measured with biochemical methods. Results: the content of MDA in liver was significantly lower in the experimental group than that in the control group about 31.2%; The activities of GSH px,CAT and Ca 2 ATPase were increased about 25.1%,49.6% and 25.4% respectively in the experimental group. The results indicate that melatonin may have an effect on clearing oxygen free radical,free calcium ion and protecting hepatic cells.