| 白介素-17在自身免疫性肝炎中的作用及其机制研究 |
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| 引用本文： | 余海静,黄加权,刘阳,余世敏,张建军.白介素-17在自身免疫性肝炎中的作用及其机制研究[J].中西医结合肝病杂志,2014(1):47-49,I0002. |
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| 作者姓名： | 余海静 黄加权 刘阳 余世敏 张建军 |
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| 作者单位： | [1]湖北省中山医院肝病科湖北武汉430033 [2]华中科技大学同济医学院附属同济医院感染科 [3]华中科技大学同济医学院附属同济医院耳鼻咽喉科 |
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| 基金项目： | 湖北省卫生厅自然科学基金（No.XF2010-10） |
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| 摘 要： | 目的：探讨IL（白细胞介素）-17在自身免疫性肝炎（autoimmune hepatitis，AIH）患者和AIH小鼠中的作用及其机制。方法：选取AIH患者和健康者各12例，分离其外周血单个核细胞（PBMC），实时定量PCR方法检测PBMC中IL-17的表达。将S-100肝抗原与弗氏完全佐剂经腹腔注入到C57BL／6小鼠体内而建立AIH小鼠模型，通过尾静脉注入IL-17中和抗体，观察小鼠肝功能变化和肝脏病理组织学变化。结果：与健康者相比，AIH患者PBMC中IL-17的表达明显增高。AIH小鼠模型中，IL-17中和抗体明显地降低了血清中ALT水平，并且减轻了小鼠肝脏炎症反应。结论：IL-17在AIH患者血清中明显升高。采用IL-17中和抗体干预后，其AIH小鼠的肝功能及肝脏损伤明显好转。证实了IL-17在AIH的发病过程中起了极其重要的作用。
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| 关 键 词： | 肝炎 自身免疫性 白细胞介素-17 外周血单个核细胞 C57BL 6小鼠 |
The role of IL-17 in pathogenosis of autoimmune hepatitis |
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| YU Hai-jing,HUANG Jia-quan,LIU Yang,ZHANG Jian-jun.The role of IL-17 in pathogenosis of autoimmune hepatitis[J].Chinese Journal of Integrated Traditonal and Western Medicine on Liver Diseases,2014(1):47-49,I0002. |
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| Authors: | YU Hai-jing HUANG Jia-quan LIU Yang ZHANG Jian-jun |
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| Institution: | 1. Department of Liver Diseases of Hubei Zhongshan Hospi- tal, Wuhan 430033, China. 2. Department of Infectious Disease, Tongfi Hospital, Tongji Medical College, Huazhong University of Science and Technology. 3. Department of Otolaryngology-Head and Neck Surgery, Tongi Hospital, Tongi Medical College, Huazhong University of Science and Technology. (Wuhan Hubei, 430030) China) |
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| Abstract: | Objective： To investigate the role of Intedeukin-17 （IL-17） in autoimmune hepatitis （AIH） . Methods： IL- l7 in the peripheral blood mononuclear cells （PBMC） of AIH patients and healthy control was measured with Real-time PCR method. To establish a mouse model of experimental autoimmune hepatitis, the syngeneic S-100 antigen emulsified in complete Freudg adjuvant was injected intraperitoneally into adult male C57 BL/6 mice. IL-17 neutralizing antibody was used to study the biological effect of IL-17 in the experimental autoimmune hepatitis. Results： The results showed that IL-17 level was significantly upregulated in AIH patients. IL-17 neutralizing antibody in vivo administration alleviated the hepatic inflammation and ALT level in the AIH model. Conclusion： It was concluded that IL-17 contributed to the development of AIH and might be a potential ther- apeutic target of AIH. |
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| Keywords: | autoimmune hepatitis interleukin-17 peripheral blood mononuclear cell C57 BL/6 mouse |
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