丹参对暴发性肝衰竭大鼠肝损伤及肝再生的影响

目的：探讨丹参对暴发性肝衰竭（FHF）大鼠肝损伤及肝再生的影响。方法：Wistar大鼠随机分为4组：正常对照组、FHF组、丹参组、促肝细胞生长素（PHGF）组。FHF、丹参组和PHGF组动物模型采用TAA皮下注射,剂量按每kg体重600mg,2次,每次间隔24h,复制FHF动物模型。丹参组和PHGF组动物除皮下注射TAA外,于实验前3天至实验结束分别于皮下注射丹参注射液1ml/100g和PHGF1ml/100g,正常对照组和FHF组大鼠同时皮下注射生理盐水1ml/100g。FHF组、丹参组和PHGF组,于第2次注射TAA后24h,随机取大鼠各8只,腹主动脉取血测肝功能。迅速取肝组织,用10%甲醛液固定,制成石蜡切片,检测肝细胞有丝分裂指数（MI）和增殖细胞核抗原（PCNA）。结果：丹参具有降低FHF大鼠ALT、AST及TBil,显著提高肝细胞MI和PCNA的作用（P〈0.05,P〈0.01）。结论：丹参具有改善FHF大鼠肝功能和促进肝细胞增殖及再生的作用。

Effect of Radix Salviae Miltiorrhizae on liver damage and liver regeneration in thioacetamide-induced acute hepatic failure in rats

Objective： To study the effect of Radix Salviae Miltiorrhizae on liver damage and liver regeneration in thioacetamide-induced acute hepatic failure in rats. Methods： The rats were randomly divided into normal group,model group,Radix Salviae Miltiorrhizae group and lyophilized bovine transfer factor control group. Medications were given 1ml /100 g respectively 3days before hypodermically injected with thioacetamide（ TAA） once a day. The rats were hypodermically injected with TAA600mg·kg-1twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the 0. 9% salt solution hypodermically. Forty-eight hours later,the blood were collected to measure ALT,AST,TBil,and at the same time,the parameters of liver regeneration were observed as following： liver mitotic index and proliferation cell nucler antigen. Results： Radix Salviae Miltiorrhizae could obviously decrease ALT,AST,TBil,improved liver mitotic index and proliferation cell nucier antigen of rats with acute hepatic failure. Comparing with model group and control group,there were significant difference（ P〈0.05 or P〈 0. 01）. Conclusion： Radix Salviae Miltiorrhizae has esistance against the rat with acute hepatic failure,improve liver regeneration.