人参皂苷Rg3对小鼠肝癌血管形成的影响

目的：探讨人参皂苷Rg3抑制人肝癌组织血管生成的机制。方法：①选用昆明种小鼠6只（雌雄各半，供传代用），昆明种小鼠140只（雌雄各半）按体重随机分为正常对照组（20只），肝癌模型组（30只），人参皂苷R船组（30只），5-FU（5-氟尿嘧啶）组（30只），人参皂苷Rg3联合5-FU组（30只）。采用小鼠肝癌细胞株H22肝内注射建立小鼠移植性肝癌模型的方法，将小鼠肝癌细胞株H22肝内注射24h后，人参皂苷Rg3组小鼠灌胃给药，5-FU组腹腔内注射给药，人参皂苷Rg3联合5-FU组小鼠灌胃和腹腔内注射给药，连续10d后处死各组全部小鼠并留取标本进行检测。②免疫组织化学染色观察各组小鼠肝组织血管内皮生长因子（VEGF）的表达。结果：5-FU组、人参皂苷Rg2组、人参皂苷Rg3联合5-FU组小鼠肝癌组织VEGF表达水平均低于肝癌模型组（均P〈0．05）；人参皂苷Rg3组和人参皂苷Rg3联合5-FU纽小鼠肝癌组织VEGF表达水平均低于5-FU组（均P〈0．05）；人参皂苷Rg3联合5-FU组小鼠肝癌组织VEGF表达水平与人参皂苷Rg3组比较，差异无显著性意义（P〉0．05）。结论：人参皂苷Rg3可抑制肝癌细胞血管生成，其可能机制是抑制VEGF表达。

Effect of ginsenoside Rg3 on expression of VEGF in mice with hepatocellular carcinoma

Objective： To study the effect of ginsengoside Rg3on the expression of VEGF in hepatocellular carcinoma in mice. Methods： ①One hundred-forty mouse of kunming species, with equal ratio of male andfemale, according to weight, were randomly divided into 5groups： normal control group （20）, HCC model group （30）, 5-FU group （30）, ginseno side Rg3group （30）, ginsenoside Rg3 combined with 5-FU （30）. H22 cells were injected to liver tissue to form transplanted HCC model in mice. After 24 hours of injection, ginsenoside Rg3 group was orally injectedinto the stomach for administration, 5-FU group was administrated by intraperitoneal injection, ginsenoside Rg3 combined with 5-FU group was orally injected into the stomach andperitonealfor administration. After 10 days of administration, all mouse were killed and collected liver tissues for de- tection. ②The expression of VEGF in liver tissues was observed by immuno-histochemical. Results ： The expression of VEGF of hepatic tissues of normal control group was significantly lower than HCC tissues of HCC model group （ P 〈 0. 05） ; the expression of VEGF of HCC tissues of 5-FU group , ginsenoside Rg3 group and ginsenoside Rg3 combined with 5-FU group were all lower than HCC model group （P 〈 0. 05 ） ; the expression of VEGF of HCC tissues of ginsenoside Rg3 combined with 5-FU group was lower than 5-FU group （ P 〈 0. 05 ） ; the expression of VEGF of HCC tissues of ginsenoside Rg3 group was lower than 5-FU group （ P 〈0. 05 ） ; In expression of VEGF of HCC tissues, there was no significant difference between gillsenoside Rg3 group and gin- senoside Rg3 combined with 5-FU group （P 〉 0. 05 ） . Conclusion： Ginsengoside Rg3 can inhibit angiogenesis of HCC , the mechanisn may be inhibit the expression of VEGF.