温阳解毒化瘀颗粒对肠源性内毒素血症大鼠肠黏膜上皮紧密连接的影响

目的：研究温阳解毒化瘀颗粒对肠源性内毒素血症（ IETM ）模型大鼠结肠黏膜上皮紧密连接的影响，探索其抗肝衰竭的作用机制。方法：将大鼠随机分为正常组、模型组、温阳解毒化瘀颗粒（实验组）和对照组4组，采用D-半乳糖胺（D-gal）腹腔注射致肝衰竭ITEM大鼠模型。正常组在腹腔注射生理盐水24h后处死，模型组、实验组、对照组分别于造模后24h、48h、72h各取6只、7只、7只大鼠处死，检测各组肝功能、内毒素、结肠黏膜上皮咬合蛋白（occludin）及肌球蛋白轻链激酶（MLCK）。结果：模型组血清ALT/AST、内毒素、 MLCK表达水平均高于正常组， occludin表达低于模型组（ P＜0.01）；实验组血清ALT/AST、内毒素、 MLCK表达水平均低于模型组， occlu-din表达高于模型组（ P＜0.05）。结论：增强结肠粘膜上皮紧密连接功能，降低内毒素的吸收是温阳解毒化瘀颗粒抗肝衰竭的作用机制之一。

Effect of Wenyang Jiedu Huayu granule on tight connection of colon muco-sain rats with intestinal endotoxemia

Objective： To investigate the effect of Wenyang Jiedu Huayu granule on tight connection of colon mucosain rats with intestinal endotoxemia （ IETM） and explore its potential mechanism of anti-hepatic failure.Methods： Rats were randomly divided into four groups： normal group , model group , Wenyang Jiedu Huayu granule group （ experimental group ） and control group.The model of hepatic failure intestinal endotoxemia was established by intraperitoneal injection of D -galactosamine.Nor-mal group were put to death twenty-four hours after intraperitoneal injection of saline.Respectively select 10 rats and put them to death from the rest groups at 24h, 48h, 72h after modeling, the liver function, endotoxin, expression of occlusal protein （oc-cludin） and myosin light chain kinase （MLCK） in intestinal epithelial were inspected.Results： In model group, serum levels of alanine/grass transaminase （ALT/AST）, endotoxin, expression of MLCK were higher than nomal group; while expression of occludin were lower than normal group （P〈0.01）; In experimental group, serum levels of alanine/grass transaminase （ALT/AST）, endotoxin expression of MLCK were lower than model group; while expression of occludin were higher than model group （P〈0.05） .Conclusion： The possible mechanisms of Wenyang Jiedu Huayu granule in anti-hepatic liver failure might be it could strengthen the tight connection of intestinal tissue , reducing the absorption of endotoxin.