电针水沟对脑出血大鼠脑血管神经调节物质影响的实验研究

目的:观察神经肽Y(NPY)和降钙素基因相关肽(CGRP)在脑出血大鼠海马结构中的表达,探讨电针"水沟"穴对脑出血大鼠脑血管神经调节物质的作用及可能机制。方法:将健康成年Wistar大鼠30只随机分为正常组、模型组、电针组,每组各10只。除正常组外,其它各组均采用胶原酶Ⅶ诱发大鼠脑区尾壳核出血模型;电针组取"水沟"穴治疗,采用连续波,频率120次/min,强度1mA,留针30min。造模麻醉清醒后立即针刺1次,以后每隔24h针刺1次,3d后取材。采用免疫组化方法检测各组大鼠海马组织NPY和CGRP表达。结果:模型组大鼠脑出血后3d,海马组织NPY免疫阳性细胞呈现高于正常组的强阳性反应(P<0.01),胞质和突起浓染呈棕褐色;而CGRP呈弱阳性反应,与正常组比较差异有显著性意义(P<0.05)。电针组大鼠海马组织NPY阳性细胞分布较稀疏,阳性反应的平均吸光度值较正常组、模型组明显减小(P<0.01);CGRP阳性细胞的平均吸光度值则较正常组和模型组增高,差异有显著性意义(P<0.01)。结论:电针"水沟"穴能够抑制大鼠脑内NPY过度表达,上调CGRP的表达,其作用可能会降低NPY的缩血管功能,增强CGRP的扩血管功能,改善脑出血后脑血管的舒缩功能紊乱,对脑组织起到保护作用。

Effect of Electroacupuncture on the Expression of Hypothalamic Neuropeptide Y and Calcitonin Gene-related Peptide in Rats with Cerebral Hemorrhage

Objective:To investigate the effect of electroacupuncture(EA)on the expression of neuropeptide Y(NPY)and calcitonin gene-related peptide(CGRP)in hippocampus tissue in cerebral hemorrhage rats so as to reveal its underlying mechanism in protecting ischemic cerebral neurons.Methods:Thirty Wistar rats were randomly divided into normal control,model and EA groups,with 10 cases in each.Cerebral hemorrhage model was duplicated by intracerebral injection of collagenase Ⅶ(0.8 μL,AP:1.0 mm,R:4.0 mm).EA(1 mA,2 Hz)was applied to "Shuigou"(GV 28)for 30 min,once again 24 h later.NPY and CGRP expression of hippocampus tissue was assayed with immunohistochemical method(SABC).Results:Compared with normal control group,OD values of hippocampal NPY immuno-reaction(IR)-positive and CGRP IR-positive neurons in model group increased obviously(P<0.01,0.05),while compared with model group the OD value of NPY IR-positive neurons in EA group decreased significantly(P<0.01),and the OD value of CGRP IR-positive neurons increased further considerably(P<0.01).Conclusion:EA of "Shuigou"(GV 28)can suppress cerebral ischemia-induced over expression of NPY,and potentiate the expression of CGRP IR-positive neurons in the hippocampus,balancing functional activities of NPY and CGRP to improve microcirculation in the brain tissue.