| CpG寡核苷酸联合Texo对小鼠结肠癌的治疗作用 |
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| 引用本文： | 刘允刚,张群,陈龙邦. CpG寡核苷酸联合Texo对小鼠结肠癌的治疗作用[J]. 医学研究生学报, 2010, 23(3): 228-232 |
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| 作者姓名： | 刘允刚 张群 陈龙邦 |
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| 作者单位： | 南京大学医学院临床学院(南京军区南京总医院)肿瘤内科,南京,210002 |
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| 基金项目： | 南京军区医药卫生科研基金(06MA124) |
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| 摘 要： | 目的肿瘤细胞分泌的外泌体(tumor-derived exosomes,Texo)是目前肿瘤免疫治疗领域的一个热点。文中主要观察小鼠结肠癌细胞CT-26分泌的Texo联合包含有未甲基化的CpG二核苷酸的寡聚脱氧核苷酸(CpG Oligodeoxynucleotides,CpG ODN)对小鼠结肠癌细胞CT-26移植瘤的治疗作用。方法使用差速离心法分离和提取CT-26细胞分泌的Texo,紫外分光光度计进行蛋白定量,透射电镜观察Texo的形态。用Texo、CpG ODN1826、CpG ODN1826+Texo(联合组)和等渗盐水(对照组)免疫BALB/c小鼠,四甲基偶氮唑蓝(MTT)法测定T细胞对CT-26细胞的体外杀伤活性。建立CT-26小鼠结肠癌皮下移植瘤模型,1周后皮下注射Texo、CpG ODN1826、CpG ODN1826+Texo以及等渗盐水,观察它们对皮下移植瘤生长的抑制作用。结果CT-26细胞分泌的Texo为直径30～100 nm的膜囊泡。CpG ODN1826+Texo联合免疫小鼠的T细胞体外对CT-26细胞的杀伤作用与单药组和对照组相比均明显增强(P<0.05)。动物实验表明,Texo和C...
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| 关 键 词： | 外泌体 CpG二核苷酸的寡聚脱氧核苷酸 结肠癌 免疫治疗 佐剂 |
Inhibitory effects of combined Texo and CpG Oligodeoxynucleotides on transplanted colorectal cancer in mouse |
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| LIU Yun-gang,ZHANG Qun,CHEN Long-bang. Inhibitory effects of combined Texo and CpG Oligodeoxynucleotides on transplanted colorectal cancer in mouse[J]. Bulletin of Medical Postgraduate, 2010, 23(3): 228-232 |
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| Authors: | LIU Yun-gang ZHANG Qun CHEN Long-bang |
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| Affiliation: | Department of Oncology;School of Medicine;Nanjing University/Nanjing General Hospital of Nanjing Military Command;PLA;Nanjing 210002;Jiangsu;China |
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| Abstract: | Objective To investigate the therapeutic effects of Texo,a tumor-derived exosome secreted by mouse colorectal cancer cell(CT-26),in combination with CpG 0ligodeoxynucleotides(CpG ODN) in a transplantation model of mouse colorectal cancer.Methods A differential centrifugation method was employed to segregate and extract Texo,which was quantitated by ultraviolet spectrophotometer and observed by transmission electron microscope.BALB/c mice were randomly divided into four groups according to the different immu... |
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| Keywords: | Exosome CpG oligodeoxynucleotides Colorectal cancer Immunotherapy Adjuvant |
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