高效液相色谱法测定天麻含片中天麻苷含量

目的：建立高效液相色谱法（HPLC）测定天麻含片中天麻苷（gastrodin）含量的方法，以期为天麻含片建立质量控制标准提供依据。方法：采用HPLC，以gastrodin为对照品，ZorbaxSB-C18键合硅胶柱（4．6mm×150mm，5μm），0．05％磷酸：乙睛（3：97）为流动相，流速1．0ml·min^-1，柱温为室温，检测波长220nm。结果：天麻苷在0．049～0．396ml^-1范围与峰面积呈良好线性关系，回归方程A=1．71×10^6＋5．864×10^7，r=0．9992（n=6）；测得2批天麻含片的天麻苷含量分别为1．0520mg／片和0．9692mg/片，平均回收率95．28％；方法精密度（RSD=0．98％，n=5）和重现性（RSD=0．78％，n=5）良好；天麻苷在4h内测定稳定RSD=1．16％。结论：该法用于测定天麻含片中天麻苷的含量，操作简便快速，结果准确可靠。

Determination of Gastrodin in Tianma Suckle Tablets by HPLC

Objectives： To establish the HPLE menthod of determining content of gastrodin in Tianma Suckle Tablets in wanting to eontrol the quality.Methods： Gastrodin was taken as standard preparation.The analytical column was ZorbaxSB-C19 .The mobile phase was aeetonitrile： 0.05% phosphoric acid solution （97： 3） .The flow rate was 1.0ml·min^-1 and the detection wavelength was 22- 0nm.Results： the gastrodin has good linear relationship in the range ot0.066 - 0.396mg ·ml^-1 and the regression equation was A = 1.71 × 10^6 ＋ 5.864× 10^7C, r = 0.9992 （ n = 6}; the content of gastrodin in two different batch were 1.0520mg/tablet and 0.9692mg/ tablet. The average recovery was 95.28 % （ RSD = 1.22 % ）, the RSD of precision was 0.98 % and repeatability RSD was 0.78 %.The stability RSD was 1.16% and gastrodin was stable within 4h. Conclusion： The method is simple, accurate and reliable. It suitable for determination of gastrodin in Tianma Suckle Tablet.