薄层色谱指纹图谱对熟地黄质量稳定性的研究

目的：为鉴别生熟地黄及考察熟地黄饮片质量的稳定性和一致性,建立了薄层色谱指纹图谱。方法：薄层板：自制的0.25mol磷酸二氢钠缓冲盐硅胶G板;展开系统：乙酸乙酯∶丙酮∶甲酸∶水（3∶5∶1∶1）,显色：苯胺-二苯胺-磷酸溶液为显色剂,85℃加热至斑点显色清晰。结果：色谱图谱及全通道扫描结果显示,10批清蒸和10批酒炖样品间的相似程度较高,饮片质量比较均一、稳定。结论：薄层色谱方法操作简单,图谱特征明显,可比性强,是一种可以快速鉴别药材真伪及考察药材质量是否稳定、一致的有效手段。

Researching on the Stability of Radix rehmanniae preparata with TLC Fingerprint

Objectives：Established TLC fingerprint of Radix rehmanniae preparata to identify Radix rehmanniae and Radix rehmanniae preparata,check the stability and consistence between different batches.Methods：Homemade 0.25m sodium dihydrogen phosphate silica gel G plate was used.Solvent system was composed of ethyl acetate∶acetone∶formic acid∶water（3∶5∶1∶1）.Aniline-diphenylamine-phosphoric acid was used as chromogenic agent,and the heating temperature at 85℃.Results：Good similarity among 10 batches of Radix rehmanniae preparata steamed and 10batches of Radix rehmanrdae preparata stewed were demonstrated by TIC finger-print images and whole channels scanning results. Conclusions： For easy operations, c ＇haracteristic chromatogram, better comparability, TLC fingerprint could used as one efficient means to identify true or false and check the stability and consistence of medicinal materials.