| 葛根提取物诱导急性非淋巴细胞性白血病细胞凋亡的实验研究 |
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| 引用本文： | 赵谢兰,齐振华,陈方平.葛根提取物诱导急性非淋巴细胞性白血病细胞凋亡的实验研究[J].湖南中医药大学学报,2000,20(3):32-34. |
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| 作者姓名： | 赵谢兰 齐振华 陈方平 |
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| 作者单位： | 湖南医科大学湘雅医院,血液科,湖南,长沙,410008 |
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| 基金项目： | 湖南省科技厅科研项目,01-951-28-1, |
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| 摘 要： | 为了探讨葛根提取物诱导白血病细胞凋亡的作用及机制。以１８例急性非淋巴细胞性白血病（ＡＮＬＬ）患者骨髓及ＨＬ－６０细胞分别与葛根提取物、高三尖杉酯碱（ＨＨＴ）、阿糖胞苷（Ａｒａ－ｃ）及ＨＡ（ＨＨＴ＋Ａｒａ－ｃ）共同培养２４ｈ,应用细胞形态学、ＤＮＡ凝胶电泳、ＤＮＡ片段百分率测定等观察以上处理对白血病细胞凋亡的影响;对其中１０例处理前后的骨髓细胞用免疫组化法进行ｂｃｌ－２、ｃ－ｍｙｃ（Ｂ细胞淋巴瘤／白
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| 关 键 词： | 葛根提取物 细胞凋亡 急性白血病 ANLL 实验研究 |
Experimental Studies on Apoptosis of Acute Nonlymphocytic Leukemia Cells Induced by Pueralia Lobata |
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| ZHAO Xielan,QI Zhenhua,CHEN Fangping.Experimental Studies on Apoptosis of Acute Nonlymphocytic Leukemia Cells Induced by Pueralia Lobata[J].Journal of Traditional Chinese Medicine University of Hunan,2000,20(3):32-34. |
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| Authors: | ZHAO Xielan QI Zhenhua CHEN Fangping |
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| Abstract: | The purpose of the study was to investigate the effects and mechanism of pueralia Lobata (PL)on the apoptosis of leukemia cells.The target leukemia cell line HL-60 and bone marrow leukemia cells from 18 acute nonlymphocytic leukemia(ANLL)patients(3M ! 7M 2 6M 3 2M 5)at presentation were studied.Apoptosis was detected by morphological observation,fluorescence assay,DNA electrophoresis,Percentage of DNA fragmentation test.It was concluded that PL could induce apoptosis in ANLL cells and HL-60 cells. |
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| Keywords: | HL-60 |
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