细胞角蛋白在肿瘤性滋养细胞的检测中的应用价值

应用免疫组化方法，采用广谱抗细胞角蛋白单克隆抗体，检测１２９例滋养细胞肿瘤（葡萄胎３０例，侵蚀性葡萄胎４１例，绒毛膜癌５８例）中肿瘤性滋养细胞内细胞角蛋白表达。结果表明，肿瘤性合体滋养细胞（ＳＴ）、中间型滋养细胞（ＩＴ）及细胞滋养细胞（ＣＴ）内细胞角蛋白的阳性表达率均为１００％。ＳＴ呈强阳性表达，其表达形式为胞浆型；ＩＴ呈中－强阳性表达，多数为胞浆型；ＣＴ呈中等强度阳性，一般为质膜型。我们认为，细胞角蛋白实为肿瘤性滋养细胞（包括转移瘤）的一种更为敏感、可靠的标志物，在滋养细胞肿瘤的病理诊断、鉴别诊断及肿瘤浸润范围的确定方面具有重要的应用价值。

Applied value of cytokeratin in the detection of neoplastic trophoblasts

The expression of cytokeratin in 129 trophoblastic tumors was studied immunohistochemically using an antibody against broad spectrum cytokeratin. The results showed that the positive rate of neoplastic syncytiotrophoblast (ST) ; intermediate trophoblast (IT) and cytotrophoblast (CT) was 100% respectively. The expression of ST showed strong positive, and plasma type; the expression of IT was middle to strong positive,most of IT was plasma type; the expression of CT appeared middle positive, and plasma membrane type. We beliyed that cytokeratin was a sensitive and reliable marker for neoplastic trophoblasts (including metastatic tumor), and had important value for pathological diagnosis, deferred diagnosis and determination of infiltrating range of trophoblastictumors.