大肠癌酶细胞化学及电镜观察

对７５例大肠癌组织中的碱性磷酸酶（ＡＫＰａｓｅ）、酸性磷酸酶（ＡＣＰａｓｅ）、琥珀酸脱氢酶（ＳＤＨａｓｅ）、葡萄糖－６－磷酸酶（Ｇ－６－Ｐａｓｅ）、焦磷酸硫胺素酶（ＴＰＰａｓｅ）活性进行了细胞化学检测及电镜观察，结果表明；ＡＫＰａｓｅ活性多呈阴性反应，ＡＣＰａｓｅ、ＳＤＨａｓｅ、Ｇ－６－Ｐａｓｅ、ＴＰＰａｓｅ四种酶活性在大肠癌明显低于大肠正常粘膜及腺瘤（Ｐ＜０．０１），在高分化癌组明显高于低分化癌组（Ｐ＜０．０５），在淋巴结无转移组明显高于有转移组（Ｐ＜０．０５），提示：对大肠癌组织同时测定ＡＣＰａｓｅ、ＳＤＨａｓｅ、Ｇ－６－Ｐａｓｅ、ＴＰＰａｓｅ活性，并在超微结构上对酶反应颗粒定位观察，能在一定程度上反映大肠癌细胞的功能状态，可以作为判断大肠癌生物学行为及预后的重要指标之一。

The study of the enzyme activities in adenocarcinoma of Iarer intestin-The Electron Histochemicol Observation

Abstract We had studied enzyne activities of AKPase, ACPase, SDHase, G-6-Pase, TPPase in 75cases of adenocarcinoma of larger intestin tissues by using the histochemical method and the electron enzyme histochemical method. Results: 1. AKPase were almost negative reaction. 2. The positive rates of ACPase, SDHase,G-6-Pase and TPPase were lover than that of normal Larger intestin mucosa and adenoma. (P<0. 01). 3. The positive rate of differentiated cancer was bigher than that of undifferentiated cancer (P<0. 05). 4. The positive rate of ACPase, SDHase, G-6-Pase in the lymph node metastasis was higher than that of no lymph node metestasis (P<0. 05). The results suggest: The activities of enzymes in adenocarcinoma of larger intestin were related to function. The histochemical investigation of ezymes in adenocarcinoma of larger intestin can be useful in determining its biologic behaviors and estimating prognostic indicator of the patients.