DNA与AgNOR在甲状腺良、恶性病变中的定量研究

本研究采用Ｆｅｕｌｇｅｎ染色和ＡｇＮＯＲ银染色，检测ＤＮＡ及ＡｇＮＯＲ物质，发现：ＤＮＡ含量Ⅲ级为甲状腺癌组＞非典型结节组＞结甲肿组＞对照组，而Ⅰ级则反之；ＡｇＮＯＲ均数为甲状腺癌组（９．２７±２：７２）＞非典型结节组（４．７８±１．６６）＞结甲肿组（３．１６±１．３８）＞对照组（３．０８±０．４１）；甲状腺癌组与各组间差异有显著意义（Ｐ＜０．０１），非典型结节组与结甲肿组、对照组间差异也有显著意义（Ｐ＜０．０１），而结甲肿组与对照组间则无差异（Ｐ＞０．０５）。作者认为Ⅲ级ＤＡＮ含量的细胞出现频率越高或／和ＡｇＮＯＲ均数越高，恶性的可能性就越大或分化程度越低；本研究提示ＡｇＮＯＲ的均数与ＤＮＡ含量具有显著的相关性，并与细胞增生的程度呈正比，故对鉴别良、恶性肿瘤及交界性病变有肯定价值。

Quantative estimation of DNA content and AgNOR count in benign and malignant thyroid Lesion

Feulgen's staining and AgNOR silver staining were employed to detect the DNA content and AgNOR count. Our findings are as follows: DNA content Grade Ⅲ in thyroid cancer groupthat in atypical nodular groupthat in noduler goiter groupthat in contrast group, while Grade Ⅰ is the opposite; AgNOR average count of the thyroid cancer group (9. 27±2. 72) of the atypical nodular group (4. 78±1. 66) of the nodular goiter group (3. 16±1. 38) contrast group (3. 08±0. 41) ; there's marked difference between the thyroid cancer group and the other groups (P0.01) and there's markable difference among the atypical nodular group, the nodular goiter group and the contrast group (P0. 01) and there's no difference between the nodular goiter group and the contrast group (P0. 05). This study suggests the marked corelation of the average count of AgNOR and DNA content and a direct ratio with cellproliferation, therefore, it is of affirmative velve in differetiating benign, malignant carcinoma and junctional lesion.