丹菟注射液对创伤性关节炎大鼠血清氧自由基的影响

背景创伤性关节炎的治疗目前仍是临床上一个棘手的问题,传统中医药治疗对创伤性关节炎有独到的优势,但其机制有待探究.目的探讨丹菟注射液对创伤性关节炎动物模型血液中一氧化氮(NO)、超氧化物歧化酶(SOD)及丙二醛水平的影响.地点和对象实验在暨南大学医学院第六附属医院内完成,对象为Wistar大鼠36只,雌雄各半,体质量(250±49)g,由湖南中医学院动物实验中心提供.设计随机对照实验.干预将36只大鼠随机分为3组丹菟注射液组、生理盐水组及正常对照组.丹菟注射液组及生理盐水组建立创伤性关节炎模型.3组动物分别予丹菟注射液和生理盐水皮下注射及不做任何处理.于第8周处死动物,采集标本.主要观察指标大鼠血一氧化氮,SOD,丙二醛水平.结果骨关节炎大鼠血中一氧化氮,SOD,丙二醛的变化结果生理盐水组分别为(63.11±20.01)mmol/L,(2023±321)nkat,(5.19±0.85)mmol/L,丹菟注射液组分别为(48.79±16.89)mmol/L,(2315±248)nkat,(4.02±1.38)mmol/L.与生理盐水组比较,丹菟注射液能降低动物血清中一氧化氮(t=2.118,P＜0.05)和丙二醛(t=2.782,P＜O.05)的水平、提升SOD(t=2.796,P＜0.05)的含量.结论丹菟注射液可通过抑制一氧化氮的表达,激活SOD,清除氧自由基,降低脂质过氧化程度,从而达到保护关节软骨,防治骨关节炎的目的.

Effect of Dantu injection on serous oxygen-derived free radicals in rat traumatic arthritis

BACKGROUND: It is still a hot potato in the clinical treatment of traumatic arthritis(TA) . Traditional Chinese medicine(TCM) has unique advantages in TA treatment; however, the mechanism is still waiting for further researches.OBJECTIVE: To discuss the effects of Dantu injection on serous levels of nitric oxide(NO), superoxide dismutase(SOD) and malondialdehyde(MDA).SETTING and PARTICIPANTS: The study was completed in the Affiliated Hospital of Jinan University Medical College. Subjects were 36 Wistar rats with half in each gender and a body mass of (250 ±49) g provided by Center for Experimental Animals, Hunan College of TCM.DESIGN: A randomized controlled study was conducted.INTERVENTIONS: A total of 36 rats were randomly divided into 3 groups:Dantu group, NS group and control group. Traumatic artuitis models were established in Dantu and NS groups. Animals in 3 groups were managed by subcutaneous injection of Dantu injection or NS or no management respectively. Animals were executed on the 8th week. Specimens were collected.MAIN OUTCOME MEASURES: The serous levels of NO, SOD and MDAin rats.RESULTS: The results of serous NO, SOD and MDA levels in arthritic rats:in NS group, they were (63. 11 ± 20. 01) mmol/L, (2023 ± 321 ) nkat, and (5.19 ±0. 85) mmol/L respectively; in Dantu group, they were (48.79 ±16.89) mmol/L, (2315 ±248) nkat, and (4.02 ± 1.28) mmol/L respectively. Compared with NS group, Dantu injection could decrease serous levelsof NO(t=2.118, P ＜0.05) and MDA(t=2.782, P ＜0.05) but increase SOD(t=2.796, P ＜0.05).CONCLUSION: Dantu injection can activate SOD through inhibiting NOexpression to eliminate oxygen-derived free radicals and decrease lipoidperoxidation, and therefore, to protect articular cartilage and preventarthritis.