含母牛分枝杆菌菌苗凝胶肺内应用的免疫效应研究

目的 观察含母牛分枝杆菌菌苗(微卡)凝胶在肺部介入应用后产生的局部及全身免疫应答效应.方法 60只日本长耳兔随机分成5组,每组12只.第1组为对照组,气管插管至末梢支气管注入生理盐水3 ml;第2组为单纯凝胶组,肺部注入卡波姆凝胶3 ml;第3、4、5组为微卡凝胶组,分别注入含3.75、7.50、9.37 mg/L母牛...

Study on immune response of Mycobacterium vaccae gel administered in lung

Objective To observe local and systemic immunity effect of Mycobacterium vaccae gel administered in lung. Methods 60 Japanese long-eared rabbits were randomly divided into five groups.Group 1 was injected with 3 ml normal sodium. Group 2 was injected with 3 ml carbomer gel. Group 3,4,5 were injected with 3 ml carbomer gel with 3.75,7.50,9.37 mg/L Mycobacterium vaccae. The peripheral blood T cell subsets and interferon-γ were tested. Results Before sacrificed,CD3 percents in group 4 and group 5 [(55.8±9.7) % and (54.5±11.8) %] were higher than that in group 1 [(43.1±3. 0) %] (all P ＜0.05),CD4 + percents in group 4 and group 5 [ (44.1 ± 5.8) % and (43.3 ± 6.2) %] were higher than that ingroup 1 [(33.5± 3.1)%] (all P ＜0.05),CD8+ percents in group 3, 4, 5 [(21.3±3.3)%,(21.1 ± 4.5) % and (20. 5 ± 4.1) % ] were lower than that in group 1 [ (28.2 ±1.7) % ] (all P ＜0.05).Interferon-γ was positive in the lung tissues injected with carbomer gel with Mycobacterium vaccae in group 4 and group 5. Conclusions Local immune stimulation can cause systemic immune response in healthy Japanese long-eared rabbits.