母牛分枝杆菌菌苗凝胶肺内应用的安全性评价

目的 观察含母牛分枝杆菌菌苗(微卡)凝胶注入肺部后的吸收和安全性,并对注入的适宜剂量和不良反应进行分析,为可用于肺结核局部免疫治疗提供依据.方法 50只日本长耳兔随机分成5组,每组10只,气管插管至末梢支气管,注入相关制剂.第1组为对照组,注人生理盐水3 ml;第2组为单纯凝胶组,注入卡波姆凝胶3 ml;第3、4、5组...

Evaluation on security of Mycobacterium vaccae gel administered in lung

Objective To provide basis for the local immune therapy of tuberculosis by observing the absorption and security of Mycobacterium vaccae (micro card) gel after administered into lung and analysing the optimum dose and side effects. Methods Fifty Japanese long-eared rabbits were randomly divided into five groups. Group 1 was injected with 3 ml normal sodium. Group 2 was injected with 3 ml carbomer gel. Group 3, 4, 5 were injected with 3 ml carbomer gel with 3.75, 7.50, 9.37 mg/L Mycobacterium vaccae. The temperature, weight, blood were tested durirg four weeks. The animals were sacrificed at the end and tissue pathology was observed. Results There was no statistical significance on temperature, weight, blood among all groups. Compared with group 1, the changes of pathology in lung of group 2 and group 3 were not obvious. There were no obvious changes on the bronchiole between group 4,5 and group 1, alveolar structure remained intact, interval mildly widened,and there was lymphocytic infiltration in interval. Conclusions Gel with Mycobacterium vaccae administered in lung has better security.