铜绿假单胞菌生物被膜的形成与环境氧浓度变化的关系研究

目的 观察氧浓度变化对铜绿假单胞菌生物被膜产生量的影响,对存在铜绿假单胞菌感染高危因素的患者是否适合高浓度的氧疗做出分析.方法 10株铜绿假单胞菌临床分离株(均来源于不同克隆株)分别在无氧(0%)、低氧(10%)、正常氧(20%)、高氧(30%、40%、50%、60%)7个不同的氧浓度下连续培养3 d后,分别用结晶紫染...

Biofilm production of Pseudomonas aeruginosa under different levels of environmental oxygen

Objective The aims of this study were to investigate the relationship between oxygen concentration and biofilm production of P. aeruginosa. Methods Ten genetically different clinieal strains of P. aeruginosa were cultured for 3 days at different levels of environmental oxygen such as no oxygen (0%), low oxygen (10%), normal oxygen (20%) and high oxygen (30%, 40%, 50%, 60%),respcetively. Then biofilm mass was quantified by crystal violet dye, silver nitrate staning to determine the time window of extra polysaccharide production under different levels of environmental oxygen. Results For quantifying the amount of biofilm mass, the highest amount of biofilm mass (1.61) was produced when the oxygen concentration was 50 %. Linear correlation analysis indicated that a significant positive correlation existed between the oxygen concentration and biofilm mass produetion (R = 0. 59). The production of extra polysaccharide by P. aeruginosa was not visualized until culture for 1 hour at ＞40%environmental oxygen concentration,2 hours for 20% and 30% oxygen concentrations, 4 hours for 10 %oxygen concentration, and 8 hours for anaerobic conditions. The time window of extra polysaccharide production was shorter with the increase of oxygen. Conclusions Hyperoxia promoted the ability of producing biofilm of P. aeruginosa which is in favor of resistance and colonization.