不同浓度屋尘螨对Der p2基因修饰的树突状细胞表面分子的影响

目的 观察不同浓度屋尘螨(HDM)对HDM过敏原Der p2基因修饰的树突状细胞(DC)表面分子的影响.方法 以未转染DC为对照,不同浓度HDM处理HDM主要抗原基因Der p2转染的DC(Der p2-DC),培养72 h后流式细胞仪检测Der p2 DC表面共刺激分子CD80、CD86和MHCⅡ.结果 加入HDM后...

Effects of house dust mites with diverse concentrations on the expression co-stimulatory molecules of Der p2-modified dendritic cell

Objective To investigate the effects of house dust mites (HDM) with diverse concentrations on the expressions of co-stimulatory molecules of HDM major antigen gene Der p2 modified dendritic cell(DC). Methods Der p2 gene modified DC(Der p2-DC) and control DC(non-transfected DC)were treated 72 h by H DM with diverse concentrations respectively. The expressions of CD80,CD86 and MHC Ⅱ ,the surface co-stimulatory molecules on Der p2-DC and control DC, were detected by FACS.Results FACS analysis showed that positive expression percentages of CD80,CD86 and MHC Ⅱ on Der p2-DC treated with HDM had no significant change,compared with Der p2-DC treated without HDM.After treated with HDM, positive expression percentages of CD80, CD86 and MHC Ⅱ on control DC increased significantly. Conclusions Specific allergen pulsing could not increase the expressions of costimulatory molecules of allergen gene modified DC.