不同剂量普萘洛尔对去卵巢大鼠骨密度和生化指标的影响

目的 观察盐酸普萘洛尔片对于去卵巢大鼠骨密度（BMD）、骨代谢标志物的影响。方法 将60大鼠随机分组：假手术组，模型空白组（生理盐水），高剂量组[20mg/（kg.d）]，中等剂量组[5mg/(kg.d）],低剂量组[1mg(/kg.d）]。假手术组在卵巢附近切除等量脂肪组织，其余各组行双侧卵巢摘除手术，术后3个月后，检测骨密度，之后，假手术组和模型空白组给予生理盐水，其余根据剂量不同每日给予大鼠灌胃相应剂量普萘洛尔水溶液。分别于第4周和第8周，检测各组BMD及骨代谢生化指标。结果 假手术组均高于各组（P＜0.01），低剂量和中等剂量组高于高剂量组和生理盐水组（P＜0.05），高剂量组与生理盐水组无统计学差异（P＞0.05）。结论 低剂量的普萘洛尔对于骨质疏松大鼠模型，可抑制骨量丢失，增加成骨细胞活性。高剂量的普萘洛尔对于骨量调节不明显。

Effect of different concentrations of propranolol on bone mineral density and bone metabolic markers in ovariectomized rats

Objective To observe the effect of propranolol hydrochloride tablets on bone mineral density (BMD) and bone metabolic markers in ovariectomized rats. Methods Sixty rats were randomly divided into 5 groups: sham group, model blank group (normal saline), high dose group (20 mg/kg.D), middle dose group (5 mg/kg.D), and low dose group (1 mg/kg.D). Rats in sham group received the operation to resect equivalent adipose tissue near the ovary. Bilateral ovariectomy was performed in rats in other groups. BMD was tested at the 3rd month after the operation. Thereafter, rats in both sham group and model blank group were given normal saline, while rats in other groups were given a gavage of propranolol aqueous solution at different concentrations according to different doses per day. BMD and bone metabolic markers were tested on the 4th and 8th week, respectively. Results The results in sham group were higher than those in the other groups (P<0.01). The results in both low dose group and middle dose group were higher than those in high dose group and normal saline group (P<0.05). The results in high dose group and normal saline group had no significant difference (P>0.05). Conclusion Low dose of propranolol can inhibit bone loss and increase the osteoblast activity in osteoporosis rats. The regulating effect of high dose of propranolol on bone mass is not obvious.