绝经后骨质疏松小鼠模型肝脏铁调素表达增加

目的 观察去卵巢小鼠铁代谢及铁调素表达量的改变，探讨铁代谢紊乱在绝经后骨质疏松中的作用机制。方法 16w龄雌性C57BL/6小鼠分为双侧去卵巢手术（OVX组）和假手术（SHAM组）两组，6w后处死，取血清、子宫、肝脏、脾脏、股骨等组织。检测股骨生物力学指标、血清骨代谢标志物、血清铁及肝脾铁的水平，肝脏铁调素的表达采用PCR技术进行检测。结果 与SHAM组相比，OVX组股骨最大弯曲应力及弹性模量降低，血清骨钙素降低，1型胶原C端肽升高，同时，血清铁降低，肝脾铁含量升高，肝脏铁调素表达升高。结论 雌激素不足增加铁调素的表达，导致组织内铁过载，与绝经后骨质疏松的发生发展有密切联系。

Increased expression of hepcidin in postmenopausal osteoporosis mice

Objective To investigate the changes of iron metabolism and hepcidin expression in ovariectomized mice, and to explore the underlying mechanism of iron metabolic disorders in postmenopausal osteoporosis. Methods Sixteen-week female C57BL/6 mice were divided into 2 groups: bilaterally ovariectomized group (OVX) and sham operation group (SHAM). All the mice were sacrificed in 6 weeks. Blood, liver, spleen, uterus, and the femur of the mice were collected. The biomechanical properties of the femur, the serum levels of bone metabolic markers, and the iron levels in the liver and the spleen were determined. The expression of hepcidin was detected using PCR methods. Results Compared with that in SHAM group, the maximal bending stress and the modulus of bending elasticity of the femur decreased significantly in OVX group. The serum levels of iron and osteocalcin decreased. The expression of hepcidin, the content of the hepatic and splenic iron, and the serum levels of C-telopeptide of type I collagen increased significantly. Conclusion Estrogen deficiency could increase the expression of hepcidin, leading to iron overload in tissues, which is closely related to the occurrence and development of postmenopausal osteoporosis.